Molecularly imprinted core-shell hybrid microspheres for the selective extraction of vanillin

• Published in: Analytical methods 2017-05, Vol.9 (19), p.2883-2889
• Main Authors: Fernandes, Rafael S, Dinc, Mehmet, Raimundo Jr, Ivo M, Mizaikoff, Boris
• Format: Article
• Language: English
• Subjects: Adsorption; Binding; Crosslinking; Extraction; Materials selection; Microspheres; Synthesis (chemistry); Vanillin
• ISSN: 1759-9660; 1759-9679
• DOI: 10.1039/c7ay00461c

Molecularly imprinted polymers (MIPs) with a core-shell structure for efficient, reliable, and selective extraction of vanillin via solid-phase extraction (SPE) and incubation methods were developed using a sol-gel process based on (3-aminopropyl)triethoxysilane (APTES) as the functional monomer, tetraethoxysilane (TEOS) as the cross-linker, and vanillin as the template. An inorganic core composed of porous (SM1) and non-porous (SM2) silica microspheres was prepared by co-condensation of TEOS and (3-aminopropyl)trimethoxysilane (APTMS) in a water-in-oil (W/O) macroemulsion. The thus synthesized materials were characterized in detail, and their molecular recognition properties and selectivity were demonstrated by evaluating their adsorption capacity and binding kinetics at imprinted (MIP) and non-imprinted (NIP) control materials. The proposed binding mechanism takes advantage of the amino groups associated with APTES interacting with the functional groups of the template molecules to form hydrogen-bonded complexes. Furthermore, synthesis conditions were optimized such that the imprinting efficiency and adsorption capacity were maximized. Finally, it was demonstrated via incubation experiments that the thus generated MIP core-shell hybrid microspheres provide rapid adsorption with high binding capacities (up to 5.64 mg g −1 ), excellent imprinting factors (IF up to 2.37), and exceptional reusability (reused >20 times). Molecularly imprinted polymers (MIPs) with a core-shell structure for efficient, reliable, and selective extraction of vanillin via solid-phase extraction (SPE) and incubation methods were developed using a sol-gel process based on (3-aminopropyl)triethoxysilane (APTES) as the functional monomer, tetraethoxysilane (TEOS) as the cross-linker, and vanillin as the template.