A wash-free SNAP-tag fluorogenic probe based on the additive effects of quencher release and environmental sensitivity

A 1,8-naphthalimide-derived fluorogenic probe was reported to label SNAP-tag fusion proteins in living cells. The probe can rapidly label a SNAP-tag and exhibit a fluorescence increase of 36-fold due to the additive effects of environment sensitivity of fluorophores and inhibition of photo-induced e...

Full description

Saved in:
Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2017, Vol.53 (48), p.6448-6451
Main Authors: Leng, Shuang, Qiao, Qinglong, Miao, Lu, Deng, Wuguo, Cui, Jingnan, Xu, Zhaochao
Format: Article
Language:English
Subjects:
Additives
Cells (biology)
Chemical compounds
Electron transfer
Fluorescence
Labels
Proteins
Sensitivity
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A 1,8-naphthalimide-derived fluorogenic probe was reported to label SNAP-tag fusion proteins in living cells. The probe can rapidly label a SNAP-tag and exhibit a fluorescence increase of 36-fold due to the additive effects of environment sensitivity of fluorophores and inhibition of photo-induced electron transfer from O -benzylguanine to the fluorophore. The labeling of intracellular proteins has been successfully achieved without a wash-out procedure.
ISSN:1359-7345
1364-548X
DOI:10.1039/c7cc01483j