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Effect of body temperature during exercise on skeletal muscle cytochrome c oxidase content
1 Department of Kinesiology, Southwestern University, Georgetown 78626; and 2 Department of Kinesiology and Health Education, The University of Texas at Austin, Austin, Texas 78712 This study determined the role of body temperature during exercise on cytochrome- c oxidase (CytOx) activity, a marke...
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| Published in: | Journal of applied physiology (1985) 2002-08, Vol.93 (2), p.526-530 |
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| cites | cdi_FETCH-LOGICAL-c473t-12c81b95ae63a43c86d1ed27f8de46577349e3d97e2dfda895d38650c6547f1a3 |
| container_end_page | 530 |
| container_issue | 2 |
| container_start_page | 526 |
| container_title | Journal of applied physiology (1985) |
| container_volume | 93 |
| creator | Mitchell, Christopher R Harris, M. Brennan Cordaro, Anthony R Starnes, Joseph W |
| description | 1 Department of Kinesiology, Southwestern
University, Georgetown 78626; and 2 Department of
Kinesiology and Health Education, The University of Texas at Austin,
Austin, Texas 78712
This study
determined the role of body temperature during exercise on
cytochrome- c oxidase (CytOx) activity, a marker of mitochondrial content, and mitochondrial heat shock protein 70 (mtHSP70), which is required for import of nuclear-coded preproteins. Male, 10-wk-old, Sprague-Dawley rats exercised identically for 9 wk in
ambient temperatures of 23°C ( n = 10), 8°C with
wetted fur ( n = 8), and 4°C with wetted fur and fan
( n = 7). These conditions maintained exercising core
temperature (T c ) at 40.4, 39.2, or 38.0°C (resting
temperature), respectively. During weeks 3-9 , exercisers ran 5 days/wk up a 6% grade at 20 m/min for 60 min. Animals
were housed at 23°C. Gastrocnemius CytOx activity in
T c =38.0°C (83.5 ± 5.5 µatoms
O · min 1 · g wet
wt 1 ) was greater than all other groups ( P |
| doi_str_mv | 10.1152/japplphysiol.00536.2001 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_pasca</sourceid><recordid>TN_cdi_proquest_miscellaneous_19153273</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>152226971</sourcerecordid><originalsourceid>FETCH-LOGICAL-c473t-12c81b95ae63a43c86d1ed27f8de46577349e3d97e2dfda895d38650c6547f1a3</originalsourceid><addsrcrecordid>eNqFkV2L1DAUhoso7rj6F7QI6lXHfDbt5bLsqrDgzXrjTcgkJzMd06YmKU7_valTWRHEqwTyvO854SmKVxhtMebk_VGNoxsPc-y82yLEab0lCOFHxSa_kgrXCD8uNo3gqBK8ERfFsxiPGWCM46fFBSaY0qZGm-LrjbWgU-ltufNmLhP0IwSVpgClmUI37Es4QdBdhNIPZfwGDpJyZT9F7aDUc_L6EHyfr6U_dUZlTvshwZCeF0-schFerOdl8eX25v76Y3X3-cOn66u7SjNBU4WJbvCu5QpqqhjVTW0wGCJsY4DVXAjKWqCmFUCMNappucmrc6RrzoTFil4Wb8-9Y_DfJ4hJ9l3U4JwawE9RCtzShrf1f0HcYk6JoBl8_Rd49FMY8ickIQS3nLGlTZwhHXyMAawcQ9erMEuM5CJJ_ilJ_pIkF0k5-XKtn3Y9mIfcaiUDb1ZARa2cDWrIAh442mDWcJ65d2fu0O0PP7oAcp3m9_MyXbZUEsnJsiz9N3k7OXcPp7REfifkaCz9Ceqnv14</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>222195446</pqid></control><display><type>article</type><title>Effect of body temperature during exercise on skeletal muscle cytochrome c oxidase content</title><source>American Physiological Society:Jisc Collections:American Physiological Society Journals ‘Read Publish & Join’ Agreement:2023-2024 (Reading list)</source><source>American Physiological Society Free</source><creator>Mitchell, Christopher R ; Harris, M. Brennan ; Cordaro, Anthony R ; Starnes, Joseph W</creator><creatorcontrib>Mitchell, Christopher R ; Harris, M. Brennan ; Cordaro, Anthony R ; Starnes, Joseph W</creatorcontrib><description>1 Department of Kinesiology, Southwestern
University, Georgetown 78626; and 2 Department of
Kinesiology and Health Education, The University of Texas at Austin,
Austin, Texas 78712
This study
determined the role of body temperature during exercise on
cytochrome- c oxidase (CytOx) activity, a marker of mitochondrial content, and mitochondrial heat shock protein 70 (mtHSP70), which is required for import of nuclear-coded preproteins. Male, 10-wk-old, Sprague-Dawley rats exercised identically for 9 wk in
ambient temperatures of 23°C ( n = 10), 8°C with
wetted fur ( n = 8), and 4°C with wetted fur and fan
( n = 7). These conditions maintained exercising core
temperature (T c ) at 40.4, 39.2, or 38.0°C (resting
temperature), respectively. During weeks 3-9 , exercisers ran 5 days/wk up a 6% grade at 20 m/min for 60 min. Animals
were housed at 23°C. Gastrocnemius CytOx activity in
T c =38.0°C (83.5 ± 5.5 µatoms
O · min 1 · g wet
wt 1 ) was greater than all other groups ( P < 0.05), exceeding sedentary ( n = 7) by 73.2%.
T c of 40.4 and 39.2°C also were higher than sedentary by
22.4 and 37.4%, respectively ( P < 0.05).
Quantification of CytOx content verified that the increased activity
was due to an increase in protein content. In extensor digitorum
longus, a nonactive muscle, CytOx was not elevated in
T c = 38.0°C. mtHSP70 was significantly elevated in
gastrocnemius of T c = 38.0°C compared with sedentary
( P < 0.05) but was not elevated in extensor digitorum longus ( P > 0.05). The data indicate that decreasing
exercise T c may enhance mitochondrial biogenesis and that
mtHSP70 expression is not dependent on temperature.
endurance exercise; mitochondrial biogenesis; mitochondrial heat
shock protein 70; glucose-regulated protein 75; rat</description><identifier>ISSN: 8750-7587</identifier><identifier>EISSN: 1522-1601</identifier><identifier>DOI: 10.1152/japplphysiol.00536.2001</identifier><identifier>PMID: 12133860</identifier><identifier>CODEN: JAPHEV</identifier><language>eng</language><publisher>Bethesda, MD: Am Physiological Soc</publisher><subject>Anatomy & physiology ; Animals ; Biological and medical sciences ; Body Temperature - physiology ; Cold Temperature ; Electron Transport Complex IV - metabolism ; Exercise ; Fundamental and applied biological sciences. Psychology ; HSP70 Heat-Shock Proteins - metabolism ; Male ; Membrane Proteins - metabolism ; Mitochondria - enzymology ; Muscle, Skeletal - physiology ; Physical Endurance - physiology ; Physical Exertion - physiology ; Proteins ; Rats ; Rats, Sprague-Dawley ; Rodents ; Striated muscle. Tendons ; Temperature ; Vertebrates: osteoarticular system, musculoskeletal system</subject><ispartof>Journal of applied physiology (1985), 2002-08, Vol.93 (2), p.526-530</ispartof><rights>2002 INIST-CNRS</rights><rights>Copyright American Physiological Society Aug 2002</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c473t-12c81b95ae63a43c86d1ed27f8de46577349e3d97e2dfda895d38650c6547f1a3</citedby><cites>FETCH-LOGICAL-c473t-12c81b95ae63a43c86d1ed27f8de46577349e3d97e2dfda895d38650c6547f1a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13814855$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12133860$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mitchell, Christopher R</creatorcontrib><creatorcontrib>Harris, M. Brennan</creatorcontrib><creatorcontrib>Cordaro, Anthony R</creatorcontrib><creatorcontrib>Starnes, Joseph W</creatorcontrib><title>Effect of body temperature during exercise on skeletal muscle cytochrome c oxidase content</title><title>Journal of applied physiology (1985)</title><addtitle>J Appl Physiol (1985)</addtitle><description>1 Department of Kinesiology, Southwestern
University, Georgetown 78626; and 2 Department of
Kinesiology and Health Education, The University of Texas at Austin,
Austin, Texas 78712
This study
determined the role of body temperature during exercise on
cytochrome- c oxidase (CytOx) activity, a marker of mitochondrial content, and mitochondrial heat shock protein 70 (mtHSP70), which is required for import of nuclear-coded preproteins. Male, 10-wk-old, Sprague-Dawley rats exercised identically for 9 wk in
ambient temperatures of 23°C ( n = 10), 8°C with
wetted fur ( n = 8), and 4°C with wetted fur and fan
( n = 7). These conditions maintained exercising core
temperature (T c ) at 40.4, 39.2, or 38.0°C (resting
temperature), respectively. During weeks 3-9 , exercisers ran 5 days/wk up a 6% grade at 20 m/min for 60 min. Animals
were housed at 23°C. Gastrocnemius CytOx activity in
T c =38.0°C (83.5 ± 5.5 µatoms
O · min 1 · g wet
wt 1 ) was greater than all other groups ( P < 0.05), exceeding sedentary ( n = 7) by 73.2%.
T c of 40.4 and 39.2°C also were higher than sedentary by
22.4 and 37.4%, respectively ( P < 0.05).
Quantification of CytOx content verified that the increased activity
was due to an increase in protein content. In extensor digitorum
longus, a nonactive muscle, CytOx was not elevated in
T c = 38.0°C. mtHSP70 was significantly elevated in
gastrocnemius of T c = 38.0°C compared with sedentary
( P < 0.05) but was not elevated in extensor digitorum longus ( P > 0.05). The data indicate that decreasing
exercise T c may enhance mitochondrial biogenesis and that
mtHSP70 expression is not dependent on temperature.
endurance exercise; mitochondrial biogenesis; mitochondrial heat
shock protein 70; glucose-regulated protein 75; rat</description><subject>Anatomy & physiology</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Body Temperature - physiology</subject><subject>Cold Temperature</subject><subject>Electron Transport Complex IV - metabolism</subject><subject>Exercise</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HSP70 Heat-Shock Proteins - metabolism</subject><subject>Male</subject><subject>Membrane Proteins - metabolism</subject><subject>Mitochondria - enzymology</subject><subject>Muscle, Skeletal - physiology</subject><subject>Physical Endurance - physiology</subject><subject>Physical Exertion - physiology</subject><subject>Proteins</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Rodents</subject><subject>Striated muscle. Tendons</subject><subject>Temperature</subject><subject>Vertebrates: osteoarticular system, musculoskeletal system</subject><issn>8750-7587</issn><issn>1522-1601</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><recordid>eNqFkV2L1DAUhoso7rj6F7QI6lXHfDbt5bLsqrDgzXrjTcgkJzMd06YmKU7_valTWRHEqwTyvO854SmKVxhtMebk_VGNoxsPc-y82yLEab0lCOFHxSa_kgrXCD8uNo3gqBK8ERfFsxiPGWCM46fFBSaY0qZGm-LrjbWgU-ltufNmLhP0IwSVpgClmUI37Es4QdBdhNIPZfwGDpJyZT9F7aDUc_L6EHyfr6U_dUZlTvshwZCeF0-schFerOdl8eX25v76Y3X3-cOn66u7SjNBU4WJbvCu5QpqqhjVTW0wGCJsY4DVXAjKWqCmFUCMNappucmrc6RrzoTFil4Wb8-9Y_DfJ4hJ9l3U4JwawE9RCtzShrf1f0HcYk6JoBl8_Rd49FMY8ickIQS3nLGlTZwhHXyMAawcQ9erMEuM5CJJ_ilJ_pIkF0k5-XKtn3Y9mIfcaiUDb1ZARa2cDWrIAh442mDWcJ65d2fu0O0PP7oAcp3m9_MyXbZUEsnJsiz9N3k7OXcPp7REfifkaCz9Ceqnv14</recordid><startdate>20020801</startdate><enddate>20020801</enddate><creator>Mitchell, Christopher R</creator><creator>Harris, M. Brennan</creator><creator>Cordaro, Anthony R</creator><creator>Starnes, Joseph W</creator><general>Am Physiological Soc</general><general>American Physiological Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TS</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20020801</creationdate><title>Effect of body temperature during exercise on skeletal muscle cytochrome c oxidase content</title><author>Mitchell, Christopher R ; Harris, M. Brennan ; Cordaro, Anthony R ; Starnes, Joseph W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c473t-12c81b95ae63a43c86d1ed27f8de46577349e3d97e2dfda895d38650c6547f1a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Anatomy & physiology</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Body Temperature - physiology</topic><topic>Cold Temperature</topic><topic>Electron Transport Complex IV - metabolism</topic><topic>Exercise</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>HSP70 Heat-Shock Proteins - metabolism</topic><topic>Male</topic><topic>Membrane Proteins - metabolism</topic><topic>Mitochondria - enzymology</topic><topic>Muscle, Skeletal - physiology</topic><topic>Physical Endurance - physiology</topic><topic>Physical Exertion - physiology</topic><topic>Proteins</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Rodents</topic><topic>Striated muscle. Tendons</topic><topic>Temperature</topic><topic>Vertebrates: osteoarticular system, musculoskeletal system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mitchell, Christopher R</creatorcontrib><creatorcontrib>Harris, M. Brennan</creatorcontrib><creatorcontrib>Cordaro, Anthony R</creatorcontrib><creatorcontrib>Starnes, Joseph W</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Physical Education Index</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of applied physiology (1985)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mitchell, Christopher R</au><au>Harris, M. Brennan</au><au>Cordaro, Anthony R</au><au>Starnes, Joseph W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of body temperature during exercise on skeletal muscle cytochrome c oxidase content</atitle><jtitle>Journal of applied physiology (1985)</jtitle><addtitle>J Appl Physiol (1985)</addtitle><date>2002-08-01</date><risdate>2002</risdate><volume>93</volume><issue>2</issue><spage>526</spage><epage>530</epage><pages>526-530</pages><issn>8750-7587</issn><eissn>1522-1601</eissn><coden>JAPHEV</coden><abstract>1 Department of Kinesiology, Southwestern
University, Georgetown 78626; and 2 Department of
Kinesiology and Health Education, The University of Texas at Austin,
Austin, Texas 78712
This study
determined the role of body temperature during exercise on
cytochrome- c oxidase (CytOx) activity, a marker of mitochondrial content, and mitochondrial heat shock protein 70 (mtHSP70), which is required for import of nuclear-coded preproteins. Male, 10-wk-old, Sprague-Dawley rats exercised identically for 9 wk in
ambient temperatures of 23°C ( n = 10), 8°C with
wetted fur ( n = 8), and 4°C with wetted fur and fan
( n = 7). These conditions maintained exercising core
temperature (T c ) at 40.4, 39.2, or 38.0°C (resting
temperature), respectively. During weeks 3-9 , exercisers ran 5 days/wk up a 6% grade at 20 m/min for 60 min. Animals
were housed at 23°C. Gastrocnemius CytOx activity in
T c =38.0°C (83.5 ± 5.5 µatoms
O · min 1 · g wet
wt 1 ) was greater than all other groups ( P < 0.05), exceeding sedentary ( n = 7) by 73.2%.
T c of 40.4 and 39.2°C also were higher than sedentary by
22.4 and 37.4%, respectively ( P < 0.05).
Quantification of CytOx content verified that the increased activity
was due to an increase in protein content. In extensor digitorum
longus, a nonactive muscle, CytOx was not elevated in
T c = 38.0°C. mtHSP70 was significantly elevated in
gastrocnemius of T c = 38.0°C compared with sedentary
( P < 0.05) but was not elevated in extensor digitorum longus ( P > 0.05). The data indicate that decreasing
exercise T c may enhance mitochondrial biogenesis and that
mtHSP70 expression is not dependent on temperature.
endurance exercise; mitochondrial biogenesis; mitochondrial heat
shock protein 70; glucose-regulated protein 75; rat</abstract><cop>Bethesda, MD</cop><pub>Am Physiological Soc</pub><pmid>12133860</pmid><doi>10.1152/japplphysiol.00536.2001</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of applied physiology (1985), 2002-08, Vol.93 (2), p.526-530 |
| issn | 8750-7587 1522-1601 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_19153273 |
| source | American Physiological Society:Jisc Collections:American Physiological Society Journals ‘Read Publish & Join’ Agreement:2023-2024 (Reading list); American Physiological Society Free |
| subjects | Anatomy & physiology Animals Biological and medical sciences Body Temperature - physiology Cold Temperature Electron Transport Complex IV - metabolism Exercise Fundamental and applied biological sciences. Psychology HSP70 Heat-Shock Proteins - metabolism Male Membrane Proteins - metabolism Mitochondria - enzymology Muscle, Skeletal - physiology Physical Endurance - physiology Physical Exertion - physiology Proteins Rats Rats, Sprague-Dawley Rodents Striated muscle. Tendons Temperature Vertebrates: osteoarticular system, musculoskeletal system |
| title | Effect of body temperature during exercise on skeletal muscle cytochrome c oxidase content |
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