A label-free electrochemical biosensor based on tubulin immobilized on gold nanoparticle/glassy carbon electrode for the determination of vinblastine

• Published in: Analytical and bioanalytical chemistry 2017-09, Vol.409 (22), p.5269-5278
• Main Authors: Haghshenas, Esmaeel, Madrakian, Tayyebeh, Afkhami, Abbas, Saify Nabiabad, Haidar
• Format: Article
• Language: English
• Subjects: Analytical Chemistry; Biochemistry; Biosensing Techniques; Biosensors; Carbodiimide; Catharanthus - chemistry; Characterization and Evaluation of Materials; Chemical properties; Chemistry; Chemistry and Materials Science; Covalence; Electrochemical analysis; Electrochemical impedance spectroscopy; Electrochemical Techniques - instrumentation; Electrochemistry; Electrodes; Equipment and supplies; Food Science; Glass - chemistry; Glassy carbon; Gold; Gold - chemistry; Humans; Identification and classification; Impedance; Laboratory Medicine; Limit of Detection; Metal Nanoparticles - chemistry; Methods; Microscopy, Electron, Scanning; Monitoring/Environmental Analysis; Nanoparticles; Plant extracts; Plant Extracts - chemistry; Production processes; Research Paper; Spectroscopy; Succinimide; Tubulin; Tubulin - chemistry; Vinblastine; Vinblastine - analysis; Vinblastine - blood; Vinblastine - chemistry
• ISSN: 1618-2642; 1618-2650
• DOI: 10.1007/s00216-017-0471-y

Vinblastine (VLB) is prescribed for a wide variety of cancers. Therefore, development of sensitive methods for early diagnosis is urgently required. In this work, a highly sensitive and label-free impedimetric biosensor was fabricated for the electrochemical detection of VLB. First, the gold nanoparticles (AuNPs) were electrodeposited on the surface of a glassy carbon electrode (GCE). 3-Mercaptopropionic acid (MPA) was self-assembled over the AuNPs. Then, tubulin (TUB), as a receptor, was covalently immobilized at the AuNPs/GCE surface via carbodiimide coupling reaction using N -(3 dimethylaminopropyl)- N′ -ethyl carbodiimide (EDC) and N -hydroxy succinimide (NHS). The step-by-step modification process was characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) in the presence of a redox probe [Fe(CN) 6 ] 3−/4− . The VLB concentration was measured through the increase of impedance values in the corresponding specific binding of VLB and TUB. The increased electron-transfer resistance ( R et ) values were proportional to the value of VLB concentrations in the range of 0.4 to 65.0 nmol L −1 with a detection limit of 8.4 × 10 −2  nmol L −1 (SN −1  = 3). The practical analytical performance of the proposed method was demonstrated by determination of VLB in plant extracts and human serum samples with satisfactory recoveries.