Direct effects of interleukin-8 on growth and functional activity of T lymphocytes

• Published in: International immunopharmacology 2017-09, Vol.50, p.178-185
• Main Authors: Meniailo, Maksim Evgenievich, Malashchenko, Vladimir Vladimirovich, Shmarov, Viacheslav Anatolievich, Gazatova, Natalia Dinislamovna, Melashchenko, Olga Borisovna, Goncharov, Andrei Gennadievich, Seledtsova, Galina Victorovna, Seledtsov, Victor Ivanovich
• Format: Article
• Language: English
• Subjects: Adaptive Immunity; Adult; Antibodies; CD18 antigen; CD25 antigen; CD28 antigen; CD3 antigen; CD4 antigen; CD4-Positive T-Lymphocytes - immunology; Cell activation; Cell Growth Processes; Cell surface; Cells, Cultured; Chains; Compartments; CXCR1; Cytokines; Effector cells; Female; Humans; Immunologic Memory; Immunological memory; Interferon; Interleukin 10; Interleukin 2; Interleukin 2 receptors; Interleukin 4; Interleukin 8; Interleukin-8 - metabolism; Lymphocyte Activation; Lymphocytes; Lymphocytes T; Magnetic separation; Male; Memory cells; Molecules; Receptors, Interleukin-8 - metabolism; Receptors, Interleukin-8A - metabolism; T-cell subset; T-Lymphocyte Subsets - immunology; Young Adult; γ-Interferon
• ISSN: 1567-5769; 1878-1705
• DOI: 10.1016/j.intimp.2017.06.023

CD3+ T-lymphocytes were isolated from the normal donors by positive magnetic separation. Activation of the T cells with particles conjugated with antibodies to CD3, СD28 and СD2 molecules led to a marked increase in T-cell production of interleukine-8 (IL-8). We present evidence that IL-8 receptor α-chain (CXCR1, CD181) is expressed on the cell surface of 13.3% T cells. Activation of T-lymphocytes resulted in significant enhancement of CD181+ cells both in naive CD4+ T cell and terminally differentiated effector CD4+ T cell compartments with concomitant reduction of CD181+ cells in effector memory CD4+ T cell subset. The level of T cell activation was assessed judging from the surface expression of CD25 (IL-2 receptor α-chain). We demonstrate that IL-8 treatment (0.01–10.0ng/ml concentration range) reduced the activation status of both CD4− and CD4+ effector memory T cells, as well as terminally differentiated effector T cells, without significantly affecting the activation of naive T cells or central memory T cells. In addition, IL-8 up-regulated IL-2 and down-regulated IL-10 production by activated T cells, with no effect on interferon-gamma (IFN-γ) and IL-4 production. Data obtained suggests the importance of IL-8 in the direct regulation of adaptive T cell reactivity. •Activation of T-lymphocytes leads to an increase in T-cell production of IL-8.•IL-8 is able to modulate IL-8 receptor expression on activated T cells.•IL-8 is capable of affecting directly TCR-mediated activation of both CD4+ and СD4− T subsets.•IL-8 is a modulator of cytokine production by activated T cells.