Partial structure and immunological properties of lipopolysaccharide from marine-derived Pseudomonas stutzeri KMM 226

• Published in: Antonie van Leeuwenhoek 2017-12, Vol.110 (12), p.1569-1580
• Main Authors: Kokoulin, Maxim S., Sokolova, Ekaterina V., Elkin, Yuriy N., Romanenko, Lyudmila A., Mikhailov, Valery V., Komandrova, Nadezhda A.
• Format: Article
• Language: English
• Subjects: Biomedical and Life Sciences; Chemical analysis; Cytokines - blood; Cytokines - metabolism; E coli; Fatty acids; Humans; Immunology; Interleukin 10; Interleukin 6; Interleukin 8; Life Sciences; Lipid A; Lipid A - chemistry; Lipid A - immunology; Lipids; Lipopolysaccharides; Lipopolysaccharides - chemistry; Lipopolysaccharides - immunology; Magnetic Resonance Spectroscopy; Marine biology; Medical Microbiology; Microbiology; O Antigens - chemistry; O Antigens - immunology; Original Paper; Plant Sciences; Pseudomonas; Pseudomonas Infections - blood; Pseudomonas Infections - immunology; Pseudomonas Infections - metabolism; Pseudomonas stutzeri; Pseudomonas stutzeri - classification; Pseudomonas stutzeri - immunology; Pseudomonas stutzeri - isolation & purification; Samples; Seawater; Seawater - microbiology; Soil Science & Conservation; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Structural analysis; Toxins; Tumor necrosis factor-α; Water analysis; Water Microbiology
• ISSN: 0003-6072; 1572-9699
• DOI: 10.1007/s10482-017-0907-6

The partial structure and immunology of the lipopolysaccharide (LPS) of Pseudomonas stutzeri KMM 226, a bacterium isolated from a seawater sample collected at a depth of 2000 m, was characterised. The O-polysaccharide was built up of disaccharide repeating units constituted by l -Rha p and d -Glc p NAc: →2)-α- l -Rha p -(1→3)-α- d -Glc p NAc-(1→. The structural analysis of the lipid A showed a mixture of different species. The major species were hexa-acylated and penta-acylated lipids A, bearing the 12:0(3-OH) in amide linkage and 10:0(3-OH) in ester linkage, while the secondary fatty acids were present only as 12:0. The presence of 12:0(2-OH) was not detected. The immunology experiments demonstrated that P. stutzeri KMM 226 LPS displayed a low ability to induce TNF-α, IL-1β, IL-6, IL-8 and IL-10 cytokine production and acted as an antagonist of hexa-acylated Escherichia coli LPS in human blood in vitro.