Effects of global change factors on fatty acids and mycosporine‐like amino acid production in Chroothece richteriana (Rhodophyta)

• Published in: Journal of phycology 2017-10, Vol.53 (5), p.999-1009
• Main Authors: Gonzalez‐Silvera, Daniel, Pérez, Sandra, Korbee, Nathalie, Figueroa, Félix L., Asencio, Antonia D., Aboal, Marina, López‐Jiménez, José Ángel, Raven, J.
• Format: Article
• Language: English
• Subjects: Acid production; Adaptation; Amino acids; Amino Acids - metabolism; Ammonium; Ammonium compounds; Ammonium Compounds - chemistry; Arachidonic acid; Biochemical composition; Cells; Chroothece; Chroothece richteriana; Climate Change; Colonies; Conductivity; Eicosapentaenoic acid; Electric Conductivity; Environmental changes; Fatty acids; Fatty Acids - metabolism; Fluidity; Global Warming; Linolenic acid; Lipid composition; Lipids; Low temperature; Membrane fluidity; Metabolism; Mycosporine-like amino acids; Polyunsaturated fatty acids; Radiation damage; Rhodophyta; Rhodophyta - metabolism; Rivers - chemistry; Spain; Temperature; Temperature effects; Ultraviolet radiation; Ultraviolet Rays
• ISSN: 0022-3646; 1529-8817
• DOI: 10.1111/jpy.12560

Under natural conditions, Chroothece richteriana synthesizes a fairly high proportion of fatty acids. However, nothing is known about how environmental changes affect their production, or about the production of protective compounds, when colonies develop under full sunshine with high levels of UV radiation. In this study, wild colonies of C. richteriana were subjected to increasing temperature, conductivity, ammonium concentrations and photosynthetically active radiation (PAR), and UV radiations to assess the potential changes in lipid composition and mycosporine‐like amino acids (MAAs) concentration. The PERMANOVA analysis detected no differences for the whole fatty acid profile among treatments, but the percentages of α‐linolenic acid and total polyunsaturated fatty acids increased at the lowest assayed temperature. The percentages of linoleic and α‐linolenic acids increased with lowering temperature. γ‐linolenic and arachidonic acids decreased with increasing conductivity, and a high arachidonic acid concentration was related with increased conductivity. The samples exposed to UVB radiation showed higher percentages of eicosapentaenoic acid and total monounsaturated fatty acids, at the expense of saturated fatty acids. MAAs accumulation increased but not significantly at the lowest conductivity, and also with the highest PAR and UVR exposure, while ammonium and temperature had no effect. The observed changes are probably related with adaptations of both membrane fluidity to low temperature, and metabolism to protect cells against UV radiation damage. The results suggest the potential to change lipid composition and MAAs concentration in response to environmental stressful conditions due to climate change, and highlight the interest of the species in future research about the biotechnological production of both compound types.