PLANT REGENERATION FROM PROTOCORM-DERIVED CALLUS OF CYPRIPEDIUM FORMOSANUM

Totipotent callus of Cypripedium formosanum, an endangered slipper orchid species, was induced from seed-derived protocorm segments on a quarter-strength Murashige and Skoog medium containing 4.52 μM 2,4-dichlorophenoxyacetic acid and 4.54 μM 1-phenyl-3-(1,2,3-thiadiazol-5-yl)-urea (thidiazuron). Th...

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Bibliographic Details
Published in:In vitro cellular & developmental biology. Plant 2003-09, Vol.39 (5), p.475-479
Main Authors: LEE, YUNG-I, LEE, NEAN
Format: Article
Language:English
Subjects:
Biological and medical sciences
Callus
callus induction
Chinese culture
Cypripedium formosanum
Embryonic stem cells
Fundamental and applied biological sciences. Psychology
Peptones
Plant physiology and development
plant regeneration
Plant roots
Plantlets
Plants
Protocols/Methods
protocorm-like body
Protocorms
Stem cells
Tissue cultures, protoplasts
Totipotent stem cells
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Summary:Totipotent callus of Cypripedium formosanum, an endangered slipper orchid species, was induced from seed-derived protocorm segments on a quarter-strength Murashige and Skoog medium containing 4.52 μM 2,4-dichlorophenoxyacetic acid and 4.54 μM 1-phenyl-3-(1,2,3-thiadiazol-5-yl)-urea (thidiazuron). This callus proliferated well and was maintained by subculturing on the same medium. On average, 13 protocorm-like bodies could be obtained from a piece of 4 mm callus after being transferred to the medium with 4.44 μM N6-benzyladenine after 8 wk of culture. The regenerated protocorm-like bodies formed shoots and roots on medium containing 1 g l−1 activated charcoal and 20 g l−1 potato homogenate. After 24 wk of culture on this medium, well-developed plantlets ready for potting were established.
ISSN:1054-5476
1475-2689
DOI:10.1079/IVP2003450