DEVISING AN IN VITRO NODULATION SYSTEM FOR TWO ACTINORHIZAL PLANTS: ALLOCASUARINA VERTICILLATA (LAM.) L. JOHNSON AND CASUARINA GLAUCA SIEB. EX SPRENG (CASUARINACEAE)

The purpose of this study was to establish an efficient in vitro nodulation device for producing actinorhizal root nodules on Allocasuarina verticillata and Casuarina glauca. Seeds from the two species were germinated aseptically and seedlings with at least two photosynthetic branchlets and a 3–5 cm...

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Published in:In vitro cellular & developmental biology. Plant 2003-09, Vol.39 (5), p.545-550
Main Authors: SY, MAME OUREYE, SAGNA, MAURICE, DIEDHIOU, SIRE, FRANCHE, CLAUDINE, AUGUY, FLORENCE, DUHOUX, EMILE
Format: Article
Language:English
Subjects:
actinorhizae
actinorhizal nodule
Agronomy. Soil science and plant productions
Allocasuarina
Allocasuarina verticillata
Biological and medical sciences
biological nitrogen fixation
calcium carbonate
Casuarina glauca
Casuarinaceae
culture media
Frankia
Fundamental and applied biological sciences. Psychology
genes
Genetic engineering applications
genetically modified organisms
Genetics and breeding of economic plants
Inoculation
Nitrogen
Nodulation
Nodules
Plant breeding: fundamental aspects and methodology
Plant growth
Plant roots
plantlets
Plants
potassium nitrate
Research Reports
Root systems
seedlings
seeds
Symbiosis
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Summary:The purpose of this study was to establish an efficient in vitro nodulation device for producing actinorhizal root nodules on Allocasuarina verticillata and Casuarina glauca. Seeds from the two species were germinated aseptically and seedlings with at least two photosynthetic branchlets and a 3–5 cm long root system were transferred into Petri dishes containing a biphasic (solid/liquid) medium. To assess the nodulation capacity, four different culture media were tested. As soon as the root system developed and spread adequately on the surface of the medium, plants were deprived of nitrogen for at least 1 wk and inoculated with the Frankia strain. The time course nodulation for A. verticillata showed that the basal Hoagland medium supplemented with CaCO3 and KNO3 was most efficient, with 83% of plantlets forming nodules, while the medium supplemented with CaCO3 reached 100% nodulation for C. glauca. This procedure can provide a valuable tool for the study of early events of actinorhizal nodulation and spatio-temporal expression of symbiotic genes in transgenic Casuarinaceae.
ISSN:1054-5476
1475-2689
DOI:10.1079/IVP2003443