Differential expression of homologous recombination DNA repair genes in the early and advanced stages of myelodysplastic syndrome

Background The high incidence of mutations and cytogenetic abnormalities in patients with myelodysplastic syndrome (MDS) suggests that defects in DNA repair mechanisms. We monitored DNA repair pathways in MDS and their alterations during disease progression. Methods Expression profiling of DNA repai...

Full description

Saved in:
Bibliographic Details
Published in:European journal of haematology 2017-10, Vol.99 (4), p.323-331
Main Authors: Valka, Jan, Vesela, Jitka, Votavova, Hana, Dostalova‐Merkerova, Michaela, Horakova, Zuzana, Campr, Vit, Brezinova, Jana, Zemanova, Zuzana, Jonasova, Anna, Cermak, Jaroslav, Belickova, Monika
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Biomarkers
Bone Marrow - pathology
CD34 antigen
Chromosome 7
Chromosome Aberrations
Cytoplasm
Deoxyribonucleic acid
DNA
DNA fingerprinting
DNA Repair
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
Female
Gene expression
Gene Expression Regulation
Homologous recombination
Humans
Male
Middle Aged
Multivariate analysis
Myelodysplastic syndrome
Myelodysplastic Syndromes - genetics
Myelodysplastic Syndromes - metabolism
Myelodysplastic Syndromes - mortality
Myelodysplastic Syndromes - pathology
Nuclei
Prognosis
RAD51
Rad51 Recombinase - genetics
Rad51 Recombinase - metabolism
Recombinational DNA Repair - genetics
Young Adult
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Background The high incidence of mutations and cytogenetic abnormalities in patients with myelodysplastic syndrome (MDS) suggests that defects in DNA repair mechanisms. We monitored DNA repair pathways in MDS and their alterations during disease progression. Methods Expression profiling of DNA repair genes was performed on CD34+ cells, and paired samples were used for monitoring of RAD51 and XRCC2 gene expression during disease progression. Immunohistochemical staining for RAD51 was done on histology samples. Results RAD51 and XRCC2 showed differential expression between low‐risk and high‐risk MDS (P
ISSN:0902-4441
1600-0609
DOI:10.1111/ejh.12920