RANTES levels as a determinant of falciparum malaria severity or recovery

• Published in: Parasite immunology 2017-09, Vol.39 (9), p.n/a
• Main Authors: Bujarbaruah, D., Kalita, M. P., Baruah, V., Basumatary, T. K., Hazarika, S., Begum, R. H., Medhi, S., Bose, S.
• Format: Article
• Language: English
• Subjects: Adult; Biomarkers - analysis; CCR5 protein; CD40 antigen; Cell activation; Chemokine CCL5 - blood; Chemokine CCL5 - metabolism; Cytokines; Cytokines - blood; Enzyme-Linked Immunosorbent Assay; Erythrocytes; Female; Flow Cytometry; Gene expression; Gene Expression Regulation; Humans; Immunomodulation; immunopathology; Malaria; Malaria, Falciparum - diagnosis; Malaria, Falciparum - parasitology; Male; Monocytes; Monocytes - immunology; Pathogenesis; Plasmodium falciparum; Plasmodium falciparum - immunology; Plasmodium sps; Polymerase chain reaction; Population studies; RANTES; Tumor necrosis factor; Young Adult
• ISSN: 0141-9838; 1365-3024
• DOI: 10.1111/pim.12452

Summary The study explored the role of differential RANTES concentrations, its receptor CCR5 expression and resulting immunomodulation in the pathogenesis and/or recovery from falciparum malaria. The study population included cases of uncomplicated malaria (UC‐M, N=128, enrolled on follow‐up basis), severe malaria (SM, N=25), and healthy controls (N=112). Serum RANTES and TNF‐α levels were evaluated by ELISA. Monocyte levels and activation profile were studied by flow cytometry. Differential mRNA expression profile was studied by real‐time PCR. Blood parasite count was evaluated by registered pathologists. RANTES concentration was significantly downregulated in SM cases compared to UC‐M (P=.046) and controls (P