Reproductive performance of immobilized cryopreserved bovine semen used for timed artificial insemination

Contents The SpermVital® technology comprises embedding of spermatozoa within an alginate gel to facilitate release of sperm cells over a prolonged period in utero after AI. The aim of this study was to examine whether the survival time of spermatozoa is extended when applying this immobilization te...

Full description

Saved in:
Bibliographic Details
Published in:Reproduction in domestic animals 2017-12, Vol.52 (6), p.1019-1024
Main Authors: Alm‐Kristiansen, AH, Dalen, G, Klinkenberg, G, Bekk, L, Thorkildsen, LT, Waterhouse, KE, Kommisrud, E
Format: Article
Language:English
Subjects:
Acrosome - physiology
Alginates
Alginic acid
animal breeding
Animals
Artificial insemination
Cattle
Cell Survival
cryobiology
Cryopreservation
Cryopreservation - veterinary
Embedding
Female
Immobilization
Insemination, Artificial - methods
Insemination, Artificial - veterinary
Lymphocytes B
Male
Mimicry
Multivariate analysis
Pregnancy
Reproduction
Reproduction (biology)
Semen
Semen Preservation - methods
Semen Preservation - veterinary
Sperm
Spermatozoa
Spermatozoa - physiology
Survival
Synchronism
Synchronization
Technology
Time Factors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Contents The SpermVital® technology comprises embedding of spermatozoa within an alginate gel to facilitate release of sperm cells over a prolonged period in utero after AI. The aim of this study was to examine whether the survival time of spermatozoa is extended when applying this immobilization technology in combination with cryopreservation. Sperm cell survival (acrosome and plasma membrane integrity) was studied in vitro for 48 hr at physiological temperature. One dose of SpermVital® (SV) semen was compared with single doses of Biladyl® (B) processed semen as well as double doses of B (B double). B double was obtained by adding a second B dose the following day, thereby mimicking double AI. Furthermore, reproductive performance applying single early timed AI (TAI) with SV following oestrus synchronization was studied in a field trial. Double insemination (TAI on two consecutive days) with B semen served as control. Number of acrosome‐intact live sperm cells decreased over time in vitro for all treatments (p  .05). However, after 48 hr, SV sperm cell survival was higher than B double (p  .05). Likelihood of pregnancy and calving in the heifer group was higher than in the cow group (p 
ISSN:0936-6768
1439-0531
DOI:10.1111/rda.13017