Evaluating of OCT-4 and NANOG was differentially regulated by a new derivative indole in leukemia cell line

• Published in: Immunology letters 2017-10, Vol.190, p.7-14
• Main Authors: Karimabad, Mojgan Noroozi, Mahmoodi, Mehdi, Jafarzadeh, Abdollah, Darehkordi, Ali, Hajizadeh, Mohammad Reza, Khorramdelazad, Hossein, Sayadi, Ahmad Reza, Rahmani, Fariba, Hassanshahi, Gholamhossein
• Format: Article
• Language: English
• Subjects: Antineoplastic Agents - pharmacology; Antitumor; Apoptosis; Cell Line, Tumor; Cell Proliferation; Cell Self Renewal; Down-Regulation; Gene Expression Regulation, Neoplastic; Humans; Indole-3-carbaldehyde; Indoles - pharmacology; NANOG; Nanog Homeobox Protein - genetics; Nanog Homeobox Protein - metabolism; Neoplastic Stem Cells - physiology; OCT4; Octamer Transcription Factor-3 - genetics; Octamer Transcription Factor-3 - metabolism; Treatment
• ISSN: 0165-2478; 1879-0542
• DOI: 10.1016/j.imlet.2017.06.012

•A significant difference in cell viability observed, when various concentrations of NI-3-C was used for 24, 48 and 72h in comparison to I3C.•Flow cytometry analysis exhibited an obviously significant augmentation in apoptotic NB4 cells.•Real Time-PCR analysis indicated that expression of NANOG/OCT4 was down-regulated compared to untreated and I3C treated cells.•Western blot analysis showed that OCT4 expression was significantly decreased in NI-3-CD treated compared to untreated and I3C treated cells. The potential exists to improve treatment through characterization of tumor stem cells and identification of therapeutic targets Using OCT-4 and NANOG genes. Here we have synthesized and investigated the potential of; New Indole-3-carbaldehyde derivative (NI-3-CD) in inhibiting the expression of self-renewal regulatory factors and cancer stem cell gene in a leukemia cell line NB4. The NB4 cells were cultured in RPMI1640 medium contained NI-3-CD and I3F (15.12–1000μg/mL) for 24, 48 and 72h. Inhibition of cell proliferation was assessed by trypan blue staining technique and MTT assay. The percentage of apoptotic cells was determined by flow cytometry analysis using Annexin V/PI apoptosis detection kit. The fold changes of NANOG/OCT4 expression against β-actin were determined by real-time-PCR technique. Western blotting analysis was also applied for evaluating the expression of NANOG/OCT4 at protein level. Data were analyzed by student t and repeated measure tests. Differences were considered significant if (P