Novel ex vivo protocol using porcine vagina to assess drug permeation from mucoadhesive and colloidal pharmaceutical systems

[Display omitted] •Ex vivo vertical model, where formulations are placed in hung porcine vaginas.•Proposed new method was capable of differentiating formulations performances.•Porcine mucosa histological structures were preserved during new assay.•Method is reliable for approaching physiological sit...

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Bibliographic Details
Published in:Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2017-10, Vol.158, p.222-228
Main Authors: Pereira, Maíra N., Reis, Thaiene A., Matos, Breno N., Cunha-Filho, Marcílio, Gratieri, Taís, Gelfuso, Guilherme M.
Format: Article
Language:English
Subjects:
Administration, Intravaginal
Animals
Colloids
Drug Delivery Systems - methods
Female
Hung vagina
Ketoconazole
Ketoconazole - chemistry
Mucoadhesive systems
Mucous Membrane - cytology
Mucous Membrane - metabolism
Nanoparticle
Nanoparticles - chemistry
Permeation protocol
Porcine vaginal mucosa
Swine
Vagina - cytology
Vagina - metabolism
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Summary:[Display omitted] •Ex vivo vertical model, where formulations are placed in hung porcine vaginas.•Proposed new method was capable of differentiating formulations performances.•Porcine mucosa histological structures were preserved during new assay.•Method is reliable for approaching physiological situation in comparative studies. Local treatment of vaginal diseases presents advantages over systemic treatments and the interaction of the drug delivery systems with the biological tissue is a key factor for a successful vaginal topical therapy. Conventional protocols for permeation studies have high variability and fail in distinguishing drug penetration from mucoadhesive or colloidal drug delivery systems from conventional formulations, as tissue interaction is normally under estimated. The protocol presented in this paper is a simplified ex vivo vertical model, in which formulations are placed in hung porcine vaginas with the objective of mimicking a condition closer to the biological circumstance, specifically considering the possible leak from the vaginal canal in the vertical position. The results indicate the proposed method was capable of differentiating formulations performances and histological evaluation showed mucosa structures are preserved during this new assay. Therefore, the ex vivo method can be considered reliable for approaching the physiological situation in comparative studies.
ISSN:0927-7765
1873-4367
DOI:10.1016/j.colsurfb.2017.07.008