Nasopharyngeal pertussis toxin IgA antibodies in the diagnosis of pertussis in Australian community patients

Nasopharyngeal aspirate (NPA) Bordetella pertussis -specific IgA antibody assay using whole-cell (WC) antigen has previously been shown to have promise in the diagnosis of patients with suspected pertussis. Recently, the use of WC assays in serum have been replaced by pertussis toxin (PT) because of...

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Bibliographic Details
Published in:European journal of clinical microbiology & infectious diseases 2017-11, Vol.36 (11), p.2259-2261
Main Authors: Norman, D., Karimi, M., Herbert, A., Beaman, M. H.
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Aged, 80 and over
Antibodies
Antibodies, Bacterial - blood
Antibodies, Bacterial - immunology
Assaying
Australia
Biomedical and Life Sciences
Biomedicine
Bordetella parapertussis - genetics
Bordetella parapertussis - immunology
Bordetella parapertussis - isolation & purification
Bordetella pertussis - genetics
Bordetella pertussis - immunology
Bordetella pertussis - isolation & purification
Child
Child, Preschool
Diagnosis
DNA, Bacterial - genetics
Female
Humans
Immunoglobulin A
Immunoglobulin A - blood
Immunoglobulin A - immunology
Infant
Infant, Newborn
Internal Medicine
Male
Medical Microbiology
Middle Aged
Mucosa
Mucous Membrane - microbiology
Nasopharynx - microbiology
Original Article
Patients
Pertussis
Pertussis toxin
Pertussis Toxin - immunology
Polymerase chain reaction
Polymerase Chain Reaction - methods
Prospective Studies
Sensitivity
Sensitivity and Specificity
Toxins
Vaccines
Whooping cough
Whooping Cough - diagnosis
Young Adult
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Summary:Nasopharyngeal aspirate (NPA) Bordetella pertussis -specific IgA antibody assay using whole-cell (WC) antigen has previously been shown to have promise in the diagnosis of patients with suspected pertussis. Recently, the use of WC assays in serum have been replaced by pertussis toxin (PT) because of specificity concerns. In this study, PT and WC B. pertussis -specific IgA antibody was assayed in 491 NPAs. Specimens also had molecular testing for the presence of B. pertussis and B. parapertussis as per the usual laboratory protocol. Positive concordance of the two serological assays was 51.2%, negative concordance was 67.5% and total concordance was 75.8%. 99 of 119 discordant specimens were resolved by utilising the B. pertussis polymerase chain reaction (PCR) result and clinical status, and yielded a sensitivity of 57.6% and a specificity 97.7% for WC, with 90.2% and 93.1%, respectively, for the PT assay ( p  
ISSN:0934-9723
1435-4373
DOI:10.1007/s10096-017-3057-x