Seasonal changes of DNA fragmentation and quality of raw and cold-stored stallion spermatozoa

In this study annual fluctuations of DNA fragmentation and quality of cold-stored equine sperm were evaluated. Ejaculates were collected weekly during one year from 15 stallions. Ejaculate volume, sperm concentration and total sperm count were determined and semen was then extended and cold-stored f...

Full description

Saved in:
Bibliographic Details
Published in:Theriogenology 2017-09, Vol.99, p.98-104
Main Authors: Wach-Gygax, L., Burger, D., Malama, E., Bollwein, H., Fleisch, A., Jeannerat, E., Thomas, S., Schuler, G., Janett, F.
Format: Article
Language:English
Subjects:
Animals
Cold Temperature
DNA Fragmentation
Horses - physiology
Male
Season
Seasons
Semen - chemistry
Semen - physiology
Semen Analysis - veterinary
Semen Preservation - methods
Semen Preservation - veterinary
Semen quality
Sperm
Sperm chromatin
Spermatozoa - physiology
Stallion
Testosterone
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In this study annual fluctuations of DNA fragmentation and quality of cold-stored equine sperm were evaluated. Ejaculates were collected weekly during one year from 15 stallions. Ejaculate volume, sperm concentration and total sperm count were determined and semen was then extended and cold-stored for 48 h. Sperm motility was evaluated by CASA before and after 24 as well as 48 h of cold storage. In addition, the percentages of sperm with intact plasma membrane and acrosome (PMAI %) and with low intracellular Ca2+ level were determined in cold-stored semen (24 h, 48 h). SCSA™ was performed to assess mean DFI, SD of DFI and % DFI in raw frozen-thawed as well as in extended sperm after 24 and 48 h of storage. The month of semen collection affected (P 
ISSN:0093-691X
1879-3231
DOI:10.1016/j.theriogenology.2017.05.025