Enhancement of Naringenin Biosynthesis from Tyrosine by Metabolic Engineering of Saccharomyces cerevisiae

Flavonoids are an important class of plant polyphenols that possess a variety of health benefits. In this work, S. cerevisiae was metabolically engineered to produce the flavonoid naringenin, using tyrosine as the precursor. Our strategy to improve naringenin production comprised three modules. In m...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2017-08, Vol.65 (31), p.6638-6646
Main Authors: Lyu, Xiaomei, Ng, Kuan Rei, Lee, Jie Lin, Mark, Rita, Chen, Wei Ning
Format: Article
Language:English
Subjects:
Flavanones - biosynthesis
Metabolic Engineering
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Tyrosine - metabolism
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Summary:Flavonoids are an important class of plant polyphenols that possess a variety of health benefits. In this work, S. cerevisiae was metabolically engineered to produce the flavonoid naringenin, using tyrosine as the precursor. Our strategy to improve naringenin production comprised three modules. In module 1, we employed a modified GAL system to overexpress the genes of the naringenin biosynthesis pathway and investigated their synergistic action. In module 2, we simultaneously up-regulated acetyl-CoA production and down-regulated fatty acid biosynthesis in order to increase the precursor supply, malonyl-CoA. In module 3, we engineered the tyrosine biosynthetic pathway to eliminate the feedback inhibition of tyrosine and also down-regulated competing pathways. It was found that modules 1 and 3 played important roles in improving naringenin production. We succeeded in producing up to ∼90 mg/L of naringenin in our final strain, which is a 20-fold increase as compared to the parental strain.
ISSN:0021-8561
1520-5118
DOI:10.1021/acs.jafc.7b02507