Autophagy regulated by lncRNA HOTAIR contributes to the cisplatin-induced resistance in endometrial cancer cells

• Published in: Biotechnology letters 2017-10, Vol.39 (10), p.1477-1484
• Main Authors: Sun, Meng-Yao, Zhu, Jian-Yong, Zhang, Chun-Yan, Zhang, Miao, Song, Ya-Nan, Rahman, Khalid, Zhang, Li-Jun, Zhang, Hong
• Format: Article
• Language: English
• Subjects: ATP Binding Cassette Transporter, Subfamily B, Member 1 - genetics; Autophagy; Beclin-1 - genetics; Cell Line, Tumor; cell lines; Cell Proliferation - drug effects; cisplatin; Cisplatin - pharmacology; Down-Regulation; Drug Resistance, Neoplasm; Endometrial Neoplasms - drug therapy; Endometrial Neoplasms - genetics; Female; Gene Expression Regulation, Neoplastic; Humans; multiple drug resistance; neoplasm cells; non-coding RNA; P-glycoproteins; RNA interference; RNA, Long Noncoding - genetics; uterine neoplasms
• ISSN: 0141-5492; 1573-6776
• DOI: 10.1007/s10529-017-2392-4

To identify whether lncRNAs (long non-coding RNA) participate in the regulation of cisplatin-resistant induced autophagy in endometrial cancer cells. Autophagy activity was significantly boosted in cisplatin-resistant Ishikawa cells, a human endometrial cancer cell line, compared with that in parental Ishikawa cells. After analyzing the overall long noncoding RNA (lncRNA) profiling, a meaningful lncRNA, HOTAIR, was identified. It was down-regulated simultaneously in cisplatin-resistant Ishikawa cells and parental Ishikawa cells treated with cisplatin. RNA interference of HOTAIR reduced the proliferation of cisplatin-resistant Ishikawa cells and enhanced the autophagy activity of cisplatin-resistant Ishikawa cells with or without cisplatin treatment, in addition, beclin-1, multidrug resistance (MDR), and P-glycoprotein (P-gp) were mediated by lncRNA HOTAIR. It is clear that lncRNAs, specifically HOTAIR, can regulate the cisplatin-resistance ability of human endometrial cancer cells through the regulation of autophagy by influencing Beclin-1, MDR, and P-gp expression.