The triterpenoid alpha, beta-amyrin prevents the impaired aortic vascular reactivity in high-fat diet-induced obese mice

• Published in: Naunyn-Schmiedeberg's archives of pharmacology 2017-10, Vol.390 (10), p.1029-1039
• Main Authors: Santos, Flávia Almeida, Carvalho, Karine Maria Martins Bezerra, Batista-Lima, Francisco José, Nunes, Paulo Iury Gomes, Viana, Ana Flávia Seraine Custódio, de Carvalho Almeida da Silva, Armenio André, da Cruz Fonseca, Said Gonçalves, Chaves, Mariana Helena, Rao, Vietla Satyanarayana, Magalhães, Pedro Jorge Caldas, de Brito, Teresinha Silva
• Format: Article
• Language: English
• Subjects: Acetylcholine; Animal tissues; Animals; Aorta; Aorta, Thoracic - drug effects; Aorta, Thoracic - physiology; Biomedical and Life Sciences; Biomedicine; Body weight; Body Weight - drug effects; Body Weight - physiology; Body weight gain; Contractility; Coronary vessels; Cyclic GMP; Diet, High-Fat - adverse effects; Dose-Response Relationship, Drug; Endothelium, Vascular - drug effects; Endothelium, Vascular - physiology; High fat diet; Hyperglycemia; Hyperlipidemia; Male; Mice; Neurosciences; Obesity; Obesity - drug therapy; Obesity - etiology; Obesity - physiopathology; Oleanolic Acid - analogs & derivatives; Oleanolic Acid - pharmacology; Oleanolic Acid - therapeutic use; Original Article; Pharmacology/Toxicology; Phenylephrine; Potassium channels; Potassium chloride; Rodents; Sodium; Sodium nitroprusside; Tetraethylammonium; Thorax; Triterpenes - pharmacology; Triterpenes - therapeutic use; Vasoconstriction - drug effects; Vasoconstriction - physiology; Vasodilation; Vasodilation - drug effects; Vasodilation - physiology
• ISSN: 0028-1298; 1432-1912
• DOI: 10.1007/s00210-017-1404-1

To characterize the protective effects of the triterpenoid mixture alpha, beta-amyrin (AMY, 20 mg/kg, during 15 days) on the reactivity of isolated aorta of high-fat diet (HFD)-induced obese mice. Male Swiss mice were fed with HFD or normal diet (ND) for 15 weeks. Contractions of thoracic aorta in response to KCl or phenylephrine (PHE) and relaxation by acetylcholine (ACh) or sodium nitroprusside (SNP) were analyzed. HFD-fed mice developed hyperglycemia, hyperlipidemia, and significant body weight gain, parameters prevented by AMY treatment. Whereas aortic contractility did not differ in response to KCl, contractions induced by PHE (1 μM) as well as relaxation induced by ACh (1–30 μM) or SNP (1 nM–0.1 mM) on PHE-contracted aorta were decreased ( p