Microsatellite markers for Hebeloma species developed from expressed sequence tags in the ectomycorrhizal fungus Hebeloma cylindrosporum

The increasing number of expressed sequence tag (EST) projects dedicated to ectomycorrhizal fungi is translating into the release of large sets of ESTs. The aim of this study was to develop and test simple sequence repeat (SSR) markers from EST databases of the model ectomycorrhizal fungus Hebeloma...

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Published in:Molecular ecology notes 2003-12, Vol.3 (4), p.659-661
Main Authors: Jany, J.L, Bousquet, J, Khasa, D.P
Format: Article
Language:English
Subjects:
alleles
DNA primers
ectomycorrhizal fungus
Expressed sequence tags
genetic markers
genetic polymorphism
Hebeloma
Hebeloma cylindrosporum
loci
microsatellite
microsatellite repeats
molecular sequence data
mycorrhizal fungi
nucleotide sequences
polymerase chain reaction
Simple sequence repeats
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description The increasing number of expressed sequence tag (EST) projects dedicated to ectomycorrhizal fungi is translating into the release of large sets of ESTs. The aim of this study was to develop and test simple sequence repeat (SSR) markers from EST databases of the model ectomycorrhizal fungus Hebeloma cylindrosporum. Six SSR markers were found to be both unambiguously scorable and polymorphic among 12 H. cylindrosporum isolates. Two SSR markers were transferable to other Hebeloma species and one marker was interestingly found to be polymorphic among seven H. crustuliniforme isolates.
doi_str_mv 10.1046/j.1471-8286.2003.00537.x
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subjects alleles
DNA primers
ectomycorrhizal fungus
Expressed sequence tags
genetic markers
genetic polymorphism
Hebeloma
Hebeloma cylindrosporum
loci
microsatellite
microsatellite repeats
molecular sequence data
mycorrhizal fungi
nucleotide sequences
polymerase chain reaction
Simple sequence repeats
title Microsatellite markers for Hebeloma species developed from expressed sequence tags in the ectomycorrhizal fungus Hebeloma cylindrosporum
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