Wood ash fertilization alters the forest humus Archaea community

The combined and separate effects of Cd and wood ash on Archaea from coniferous forest humus were studied in a microcosm experiment. Nonmetric multidimensional scaling of the denaturing gradient gel analysis of polymerase chain reaction amplified 0.9 kb 16S ribosomal DNA fragments revealed changes i...

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Bibliographic Details
Published in:Soil biology & biochemistry 2004, Vol.36 (1), p.199-201
Main Authors: Yrjälä, Kim, Katainen, Riikka, Jurgens, German, Saarela, Ulla, Saano, Aimo, Romantschuk, Martin, Fritze, Hannu
Format: Article
Language:English
Subjects:
16S rDNA
Agronomy. Soil science and plant productions
Archaea
Biological and medical sciences
cadmium
community structure
Coniferous forest humus
coniferous forests
Crenarchaea
DGGE
forest soils
Fundamental and applied biological sciences. Psychology
gel electrophoresis
polymerase chain reaction
pumice
ribosomal DNA
Sequencing
Soil and water pollution
soil bacteria
soil organic matter
Soil science
Wood ash
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Summary:The combined and separate effects of Cd and wood ash on Archaea from coniferous forest humus were studied in a microcosm experiment. Nonmetric multidimensional scaling of the denaturing gradient gel analysis of polymerase chain reaction amplified 0.9 kb 16S ribosomal DNA fragments revealed changes in archaeal communities due to the ash treatments. Cd with or without ash did not further influence the result. Representatives of the ash and control communities were cloned, grouped by restriction fragment length polymorphism analysis and finally sequenced. All sequences belonged to non-thermophilic Crenarchaea.
ISSN:0038-0717
1879-3428
DOI:10.1016/j.soilbio.2003.09.003