Comparative effects of vitamin D and methylprednisolone against ischemia/reperfusion injury of rabbit spinal cords

Recent studies have demonstrated the neuroprotective and immunomodulatory effects of 1,25-dihydroxyvitamin D3 (calcitriol), but no previous study has examined these effects on spinal cord ischemia/reperfusion (I/R) injury. Therefore, the present study aimed to evaluate whether calcitriol protects th...

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Bibliographic Details
Published in:European journal of pharmacology 2017-10, Vol.813, p.50-60
Main Authors: Gürer, Bora, Karakoç, Abdullah, Bektaşoğlu, Pınar Kuru, Kertmen, Hayri, Kanat, Mehmet Ali, Arıkök, Ata Türker, Ergüder, Berrin İmge, Sargon, Mustafa Fevzi, Öztürk, Özden Çağlar, Çelikoğlu, Erhan
Format: Article
Language:English
Subjects:
Animals
Calcitriol
Caspase 3 - metabolism
Catalase - metabolism
Disease Models, Animal
Ischemia/reperfusion injury
Male
Malondialdehyde - blood
Methylprednisolone
Methylprednisolone - pharmacology
Methylprednisolone - therapeutic use
Neuroprotection
Peroxidase - metabolism
Rabbits
Reperfusion Injury - blood
Reperfusion Injury - complications
Reperfusion Injury - drug therapy
Reperfusion Injury - metabolism
Spinal cord injury
Spinal Cord Ischemia - complications
Vitamin D
Vitamin D - pharmacology
Vitamin D - therapeutic use
Xanthine Oxidase - blood
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Summary:Recent studies have demonstrated the neuroprotective and immunomodulatory effects of 1,25-dihydroxyvitamin D3 (calcitriol), but no previous study has examined these effects on spinal cord ischemia/reperfusion (I/R) injury. Therefore, the present study aimed to evaluate whether calcitriol protects the spinal cord from I/R injury. Rabbits were randomized into four groups of eight animals: group 1 (laparotomy control), group 2 (ischemia control), group 3 (30mg/kg intraperitoneal methylprednisolone at surgery), and group 4 (0.5μg/kg, intraperitoneal calcitriol for 7 days before I/R injury). The rabbits in the laparotomy control group underwent laparotomy only, whereas all rabbits in the other groups were subject to spinal cord ischemia by aortic occlusion for 20min, just caudal to the renal artery. Malondialdehyde and catalase levels, myeloperoxidase and xanthine oxidase activities, and caspase-3 concentrations were analyzed. Finally, histopathological, ultrastructural, and neurological evaluations were performed. After I/R injury, increases in malondialdehyde levels, myeloperoxidase and xanthine oxidase activities, and caspase-3 concentrations were found (p
ISSN:0014-2999
1879-0712
DOI:10.1016/j.ejphar.2017.07.028