Characterization of the complete mitochondrial genome of Acanthoscelides obtectus (Coleoptera: Chrysomelidae: Bruchinae) with phylogenetic analysis

• Published in: Genetica 2017-10, Vol.145 (4-5), p.397-408
• Main Authors: Yao, Jie, Yang, Hong, Dai, Renhuai
• Format: Article
• Language: English
• Subjects: Animal Genetics and Genomics; Animals; Bats; Biomedical and Life Sciences; Clover; Coleoptera; Coleoptera - genetics; DNA, Mitochondrial; Evolutionary Biology; Genes; Genome, Insect; Genome, Mitochondrial; Genomes; Helices; Human Genetics; Insect Proteins - genetics; Life Sciences; Male; Microbial Genetics and Genomics; Mitochondria; Mitochondrial DNA; Nucleic Acid Conformation; Original Paper; Phylogenetics; Phylogeny; Plant Genetics and Genomics; RNA, Ribosomal - genetics; RNA, Transfer - genetics; rRNA; tRNA Ser
• ISSN: 0016-6707; 1573-6857
• DOI: 10.1007/s10709-017-9975-9

Acanthoscelides obtectus is a common species of the subfamily Bruchinae and a worldwide-distributed seed-feeding beetle. The complete mitochondrial genome of A. obtectus is 16,130 bp in length with an A + T content of 76.4%. It contains a positive AT skew and a negative GC skew. The mitogenome of A. obtectus contains 13 protein-coding genes (PCGs), 22 tRNA genes, two rRNA genes and a non-coding region (D-loop). All PCGs start with an ATN codon, and seven ( ND3, ATP6, COIII, ND3, ND4L, ND6 , and Cytb ) of them terminate with TAA, while the remaining five ( COI, COII, ND1, ND4 , and ND5 ) terminate with a single T, ATP8 terminates with TGA. Except tRNA Ser , the secondary structures of 21 tRNAs that can be folded into a typical clover-leaf structure were identified. The secondary structures of lrRNA and srRNA were also predicted in this study. There are six domains with 48 helices in lrRNA and three domains with 32 helices in srRNA . The control region of A. obtectus is 1354 bp in size with the highest A + T content (83.5%) in a mitochondrial gene. Thirteen PCGs in 19 species have been used to infer their phylogenetic relationships. Our results show that A. obtectus belongs to the family Chrysomelidae (subfamily-Bruchinae). This is the first study on phylogenetic analyses involving the mitochondrial genes of A. obtectus and could provide basic data for future studies of mitochondrial genome diversities and the evolution of related insect lineages.