Adjuvantation of inactivated Foot and Mouth Disease Virus vaccine with IL-15 expressing plasmid improves the immune response in Guinea Pigs

• Published in: Biologicals 2017-09, Vol.49, p.23-27
• Main Authors: Nagaraj, Vijay, John, Lijo, Bharatiraja, Singaram, Dechamma, Hosur Joyappa, Reddy, Golla Ramalinga
• Format: Article
• Language: English
• Subjects: Adjuvants, Immunologic - genetics; Adjuvants, Immunologic - pharmacology; Animals; B-Lymphocytes - immunology; Bovine Interleukin 15 (bIL-15); Cattle; CHO Cells; Cricetulus; Foot and Mouth Disease Virus; Foot-and-Mouth Disease Virus - chemistry; Foot-and-Mouth Disease Virus - immunology; Guinea Pigs; Immune response; Immunity, Cellular - drug effects; Immunity, Cellular - genetics; Interleukin-15 - genetics; Interleukin-15 - immunology; Interleukin-15 - pharmacology; Plasmids - genetics; Plasmids - pharmacology; PLG micro particles; T-Lymphocytes - immunology; Vaccines, Inactivated - chemistry; Vaccines, Inactivated - immunology; Vaccines, Inactivated - pharmacology; Viral Vaccines - chemistry; Viral Vaccines - immunology; Viral Vaccines - pharmacology
• ISSN: 1045-1056; 1095-8320
• DOI: 10.1016/j.biologicals.2017.07.005

Foot and mouth disease is a highly contagious disease affecting cloven footed animals. Vaccination using inactivated virus is followed to control the disease. As the immune response conferred by the inactivated vaccine is short lived, there is a need for an alternate vaccine with increased duration of immunity. Inclusion of adjuvant which enhances B and T cell responses is one of the strategies to increase the duration of immune responses of the vaccine. Interleukin 15 is one such a cytokine which improves the cell mediated immune response and also involved in the maintenance of memory T and B cells. In the present communication, we evaluated the role of bovine IL-15 as an adjuvant to inactivated FMD vaccine in guinea pig model. Animals injected with FMD inactivated vaccine and IL-15 plasmid showed improved levels of neutralizing antibodies which were maintained up to 6 months (as the level of neutralizing antibodies is more >1.5 which is considered to give protection). Increased Th1 and Th2 responses (by measuring the level of IL-4 and IFN- gamma responses) were seen in IL-15 adjuvanted guinea pigs compared to animals injected with inactivated vaccine alone.