Genetic association of pro-inflammatory cytokine gene polymorphisms with coronary artery disease (CAD) in a North Indian population

• Published in: Gene 2017-09, Vol.628, p.301-307
• Main Authors: Mastana, Sarabjit, Prakash, Swayam, Akam, Elizabeth C., Kirby, Melissa, Lindley, Martin R., Sinha, Nakul, Agrawal, Suraksha
• Format: Article
• Language: English
• Subjects: Adult; Alleles; Analysis of Variance; Biomarkers; CAD; Case-Control Studies; Coronary Artery Disease - blood; Coronary Artery Disease - genetics; Cytokines - genetics; Female; Gene Frequency; Genetic Predisposition to Disease; Genotype; Humans; IFN-ɣ + 874 (T/A) (rs2430561); IL-6 − 174 (G/C) (rs1800795); IL6 − 597 (G/A) (rs1800797); India; Male; Middle Aged; North India; Odds Ratio; Polymorphism, Single Nucleotide; Risk Factors; TNF-α − 308 (G/A) (rs1800797); TNF-β + 252 (A/G) (rs909253)
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/j.gene.2017.07.050

Cytokines regulate the expression of inflammatory molecules which destabilize the atheromatic plaques. This study focuses on studying the association of inflammatory cytokine polymorphisms like TNF-α −308 (G/A), TNF-β +252 (A/G), IL-6 −174 (G/C) and IL-6 −597 (G/A), and IFN-ɣ +874 (T/A) with coronary artery disease (CAD) among north Indian patients. 143 CAD and 137 normal healthy controls were recruited in this study. DNA extraction was carried out by high salting out method. TNF-α −308 (G/A) (rs1800797), TNF-β +252 (A/G) (rs909253), IL-6 −174 (G/C) (rs1800795), IL6 −597 (G/A) (rs1800797), and IFN-ɣ +874 (T/A) (rs2430561) SNPs were genotyped by TaqMan®SNP genotyping assays. Different statistical analyses were performed using SPSS v 22.0 and SNPStats. p≤0.05 was considered significant. Significant risk association with CAD was found for TNF-α −308 (G/A) “A” allele (OR=5.6, CI 1.8–17.4, p=0.001) and TNF-β +252 (A/G) “G” allele (OR=3.4, CI=1.9–6.0, p