Lipoxygenase Inhibitors Nordihydroguaiaretic Acid and Fungus Lecanicillum lecanii Extract Induce Death of Lymphoid Leukemia Cells

We studied the effect of lipoxygenase inhibitors nordihydroguaiaretic acid (NDGA) and fungus Lecanicillum lecanii extract on lymphatic leukemia P388 cells. The cells grown in the abdominal cavity of DBA2 mice for 7 days were transferred into a nutrient medium. The effect of lipoxygenase inhibitors w...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2017-07, Vol.163 (3), p.330-333
Main Authors: Bibikova, M. V., Spiridonova, N. A., Korystova, A. F., Kublik, L. N., Levitman, M. Kh, Shaposhnikova, V. V., Korystov, Yu. N.
Format: Article
Language:English
Subjects:
Animals
Antineoplastic Agents - pharmacology
Apoptosis
Apoptosis - drug effects
Biomedical and Life Sciences
Biomedicine
Cell Biology
Cell Nucleus - drug effects
Cell Nucleus - metabolism
Cell Nucleus - pathology
Cell Nucleus - ultrastructure
Cell number
Complex Mixtures - pharmacology
Cordyceps - chemistry
Deoxyribonucleic acid
DNA
DNA damage
DNA, Neoplasm - antagonists & inhibitors
DNA, Neoplasm - biosynthesis
Fungi
Humans
Inhibitory Concentration 50
Internal Medicine
Laboratory Medicine
Leukemia
Lipoxygenase
Lipoxygenase Inhibitors - pharmacology
Lymphatic leukemia
Lymphocytes - drug effects
Lymphocytes - metabolism
Lymphocytes - pathology
Masoprocol - pharmacology
Mice
Mice, Inbred DBA
Nordihydroguaiaretic acid
Nuclei
Pathology
Rodents
Tumor Cells, Cultured
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Summary:We studied the effect of lipoxygenase inhibitors nordihydroguaiaretic acid (NDGA) and fungus Lecanicillum lecanii extract on lymphatic leukemia P388 cells. The cells grown in the abdominal cavity of DBA2 mice for 7 days were transferred into a nutrient medium. The effect of lipoxygenase inhibitors was evaluated by changes in cell number, trypan blue staining, nucleus damage, and changes in cell distribution by DNA content after 22-h incubation. NDGA and fungus extract induced apoptotic death of lymphatic leukemia cells, which was seen from nucleus damage and reduced DNA content in cells. IC 50 for NDGA and fungus extract was 0.66 and 5.5 μg/ml, respectively.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-017-3796-9