Proteomic Analysis of Synovial Fluid Obtained From a Dog Diagnosed With Idiopathic Immune-Mediated Polyarthritis

This case study is to report the proteins detected by proteomic analysis of synovial fluid from a dog diagnosed with idiopathic immune-mediated polyarthritis, and to compare it with healthy dogs. Synovial fluid was collected via arthrocentesis from a dog diagnosed with immune-mediated polyarthritis....

Full description

Saved in:
Bibliographic Details
Published in:Topics in companion animal medicine 2017-03, Vol.32 (1), p.24-27
Main Authors: Tan, Wei Miao, Lau, Seng Fong, Ajat, Mokrish, Mansor, Rozaihan, Abd Rani, Puteri Azaziah Megat, Rahmad, Norasfaliza Binti
Format: Article
Language:English
Subjects:
2-dimensional gel electrophoresis
Abundance
Actin
Animals
Arthritis
Arthritis - blood
Arthritis - immunology
Arthritis - veterinary
Biochemistry
Biomarkers
C4b-binding protein
Case studies
Case-Control Studies
Cellular biology
Complement component C4b-binding protein
Cytokines
Diagnosis
Dog Diseases - immunology
Dog Diseases - metabolism
Dogs
Electrophoresis, Gel, Two-Dimensional - veterinary
Gel electrophoresis
Gels
immune-mediated polyarthritis
Immunoglobulins
Infections
Inflammation
Isoelectric focusing
Keratin
MALDI-TOF/MS
Medical research
Polyarthritis
Precipitation
Proteins
proteomics
Proteomics - methods
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - veterinary
Spots
Studies
Synovial fluid
Synovial Fluid - chemistry
Synovial Fluid - immunology
Wolves
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This case study is to report the proteins detected by proteomic analysis of synovial fluid from a dog diagnosed with idiopathic immune-mediated polyarthritis, and to compare it with healthy dogs. Synovial fluid was collected via arthrocentesis from a dog diagnosed with immune-mediated polyarthritis. Protein precipitation was performed on the synovial fluid, followed by isoelectric focusing and 2-dimensional gel electrophoresis. The spots on the 2-dimensional gels were analyzed using MALDI-TOF/MS. The results were then analyzed against the MASCOT database. The results from the proteomic analysis revealed an abundance of several types of immunoglobulins together with the presence of complement C4b-binding protein alpha chain. Actin and keratin were also among the proteins detected. Proteomic studies, facilitate a better understanding of the different levels of proteins expressed during disease activity. Potential disease biomarkers can aid in the diagnosis of disease, as well as help in monitoring treatment efficacy and providing prognosis for the patient.
ISSN:1938-9736
1946-9837
1876-7613
DOI:10.1053/j.tcam.2017.05.002