Detection of selected arboviral infections in patients with history of persistent fever in Pakistan

Surveillance is a valuable tool for understanding prevailing and previously undiagnosed infections in a geographic area. We examined 480 archived serum samples from patients with history of persistent fever (>40°C, 60–72h) who were referred to hospitals in Rawalpindi/Islamabad, Lahore, and Faisal...

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Bibliographic Details
Published in:Acta tropica 2017-12, Vol.176, p.34-38
Main Authors: Yaqub, Tahir, Shabbir, Muhammad Zubair, Mukhtar, Nadia, Tahir, Zarfishan, Abbas, Tariq, Amir, Ehab, Defang, Gabriel
Format: Article
Language:English
Subjects:
Adult
Antibodies, Viral - blood
Antigens, Viral - blood
Chikungunya virus
Enzyme-Linked Immunosorbent Assay
Female
Hemorrhagic Fevers, Viral - genetics
Hemorrhagic Fevers, Viral - immunology
Humans
Male
Middle Aged
Pakistan
Pakistan - epidemiology
Population Surveillance
Real-Time Polymerase Chain Reaction
Seasons
Sex Factors
Surveillance
Viral haemorrhagic fever
West Nile virus
Young Adult
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Summary:Surveillance is a valuable tool for understanding prevailing and previously undiagnosed infections in a geographic area. We examined 480 archived serum samples from patients with history of persistent fever (>40°C, 60–72h) who were referred to hospitals in Rawalpindi/Islamabad, Lahore, and Faisalabad districts for dengue antibody detection in 2014-15. Each sample was processed for detection of antigens and seroconversion, using real-time polymerase chain reaction and enzyme linked immunosorbent assay, respectively, against dengue haemorrhagic fever (DHF) virus serotypes 1–4, West Nile virus fever (WNVF), Crimean-Congo haemorrhagic fever (CCHF), and Chikungunya virus (CGV). The presence of antigens and antibodies to at least one of the studied viral haemorrhagic fevers (VHFs) was detected in 465 (96.8%, 95% CI: 94.9–98.1) and 442 samples (92.1%, 95% CI: 89.3–94.2), respectively. No sera were found positive to CCHF. There was a significant association between gender and positivity to at least one of the VHFs (χ2=8.12, df=1, p
ISSN:0001-706X
1873-6254
DOI:10.1016/j.actatropica.2017.07.019