Effect of acaricides on the activity of a Boophilus microplus glutathione S-transferase

In the present study, we report the effect of several acaricides on the enzyme activity of a Boophilus microplus recombinant glutathione S-transferase (rGST). GST was expressed in Escherichia coli and was purified with glutathione (GSH) affinity column chromatography. The kinetic constants were dete...

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Bibliographic Details
Published in:Veterinary parasitology 2004-01, Vol.119 (2), p.237-245
Main Authors: da Silva Vaz, Itabajara, Torino Lermen, Tiago, Michelon, André, Sanchez Ferreira, Carlos Alexandre, Joaquim de Freitas, Daniela Reis, Termignoni, Carlos, Masuda, Aoi
Format: Article
Language:English
Subjects:
Acaricides
Animals
Blotting, Western
Boophilus microplus
Chromatography, Affinity
Dinitrochlorobenzene - metabolism
Drug resistance
Escherichia coli - genetics
Female
Glutathione S-transferase
Glutathione Transferase - antagonists & inhibitors
Glutathione Transferase - metabolism
Insecticides - pharmacology
Ixodidae
Ixodidae - enzymology
Ixodidae - metabolism
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
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Summary:In the present study, we report the effect of several acaricides on the enzyme activity of a Boophilus microplus recombinant glutathione S-transferase (rGST). GST was expressed in Escherichia coli and was purified with glutathione (GSH) affinity column chromatography. The kinetic constants were determined by reacting GST with the substrates 1-chloro-2,4-dinitrobenzene (CDNB) and glutathione. We report the effect of several acaricides on the enzyme activity of rGST. Some acaricides (ethion, amitraz, chlorpyrifos, DDT, cypermethrin, diazinon, ivermectin, deltamethrin and flumethrin) inhibited rGST. Contrarily, coumaphos had an activating effect. Although the accurate mechanisms of the B. microplus resistance to acaricides remain elusive, this work helps in understanding how acaricides can interact with GST.
ISSN:0304-4017
1873-2550
DOI:10.1016/j.vetpar.2003.11.004