Phosphorus protects cardiac tissue by modifying the immune response in rats infected by Trypanosoma cruzi

• Published in: Cytokine (Philadelphia, Pa.) Pa.), 2018-02, Vol.102, p.102-106
• Main Authors: Ferreira, Érika Cristina, Ciupa, Larissa, Portocarrero, Angela Rigo, Brustolin, Camila Fernanda, Massini, Paula Fernanda, Aleixo, Denise Lessa, de Araújo, Silvana Marques
• Format: Article
• Language: English
• Subjects: Animals; Cardiotonic Agents - pharmacology; Chagas Disease - drug therapy; Chagas Disease - immunology; Chagas Disease - pathology; Cytokines; Disease Models, Animal; Heart - drug effects; Heart - parasitology; Homeopathy; IFN-γ; Interferon-gamma - blood; Male; Myocardium - immunology; Myocardium - pathology; Phosphorus; Phosphorus - pharmacology; Rats; Rats, Wistar; TNF-α; Trypanosoma cruzi; Trypanosoma cruzi - immunology; Tumor Necrosis Factor-alpha - blood
• ISSN: 1043-4666; 1096-0023
• DOI: 10.1016/j.cyto.2017.07.017

•Phosphorus 13cH promotes beneficial effects in murine infection by T. cruzi.•The Treatment beneficially modulates as inflammatory cytokines IFN-γ e TNF-α.•The drug reduces inflammation of cardiac tissue in Wister rats infected with T. cruzi. Aim: This study evaluates and correlates the number of myocarditis focuses and production of cytokines in Rattus norvegicus (Wistar lineage), experimentally infected with T. Cruzi and treated with Phosphorus. Methods: In two blind, controlled and randomized trials, 53 45-day-old, male animals were allocated into groups Control (n=24): Control group infected and treated with 7% hydroalcoholic solution, the preparation vehicle of the test medication; and Phosphorus (n=24 on days 0, 5, 10 and 24 after infection): group infected and treated with Phosphorus 13cH, diluted 10−26 and dynamized (test medication). The animals were inoculated intraperitoneally with 5×106 blood trypomastigotes of T. cruzi-Y strain. The medication was administered overnight (16 consecutive hours), diluted in water (1mL/100mL) in amber water bottles. The animals were treated 2days before and 2, 4, and 6days after infection. Enumeration of inflammatory foci in cardiac tissue (Hematoxylin-Eosin) and dosage of cytokines TNF-α and IFN-γ in the serum were performed on days 0, 5, 10 and 24 after infection, using three animals/group. Mann-Whitney, Friedman ANOVA, Spearman correlation (p