Pathway discovery using transcriptomic profiles in adult-onset severe asthma

• Published in: Journal of allergy and clinical immunology 2018-04, Vol.141 (4), p.1280-1290
• Main Authors: Hekking, Pieter-Paul, Loza, Matt J., Pavlidis, Stelios, de Meulder, Bertrand, Lefaudeux, Diane, Baribaud, Fred, Auffray, Charles, Brinkman, Paul, Lutter, Rene, Bansal, Aruna T., Sousa, Ana R., Bates, Steve A., Pandis, Yannis, Fleming, Louise J., Shaw, Dominique E., Fowler, Stephen J., Meiser, Andrea, Sun, Kai, Corfield, Julie, Howarth, Peter H., Bel, Elisabeth H., Adcock, Ian M., Chung, Kian Fan, Djukanovic, Ratko, Adcock, I.M., Auffray, C., Bakke, P., Bansal, A.T., Baribaud, F., Bates, S., Bel, E.H., Bigler, J., Bisgaard, H., Boedigheimer, M.J., Bønnelykke, K., Brandsma, J., Bucchioni, E., Burg, D., Caruso, M., Chanez, P., Chung, F.K., Compton, C.H., Corfield, J., D'Amico, A., Dahlen, S.E., De Meulder, B., Erpenbeck, V.J., Erzen, D., Fichtner, K., Fitch, N., Formaggio, E., Frey, U., Gahlemann, M., Geiser, T., Hashimoto, S., Haughney, J., Hedlin, G., Hohlfeld, J.M., Holweg, C., Horváth, I., Howarth, P., Knowles, R., Krug, N., Lefaudeux, D., Loza, M.J., Lutter, R., Manta, A., Matthews, J.G., Mazein, A., Middelveld, R.J.M., Mores, N., Myles, D., Pahus, L., Pandis, I., Pavlidis, S., Powel, P., Praticò, G., Valls, M Puig, Rao, N., Riley, J., Roberts, G., Rowe, A., Sandström, T., Seibold, W., Selby, A., Sigmund, R., Singer, F., Skipp, P.J., Sousa, A.R., Sterk, P.J., Sun, K., Thornton, B., van Geest, M., Vestbo, J., Vissing, N.H., Wagener, A.H., Wagers, S.S., Weiszhart, Z., Wheelock, C.E., Wilson, S.J.
• Format: Article
• Language: English
• Subjects: Adult; Adult-onset asthma; Age; Age of Onset; Asthma; Asthma - genetics; Asthma - immunology; Biopsy; Children; Cross-Sectional Studies; Data collection; Enrichment; eosinophils; Female; Gene expression; Gene Expression Profiling; gene set variation analysis; Genetic Markers; Humans; ILC3; Inflammation; Leukocytes (eosinophilic); Lungs; Lymphoid cells; Male; Mast cells; mechanisms; Medical treatment; Middle Aged; Neutrophils; Oligonucleotide Array Sequence Analysis; Patients; Phenotype; phenotyping; Quality control; Quality of life; Respiratory tract; Respiratory tract diseases; Ribonucleic acid; RNA; severe asthma; Severity of Illness Index; Sputum; Statistical analysis; Statistical methods; Transcriptome - immunology; transcriptomics
• ISSN: 0091-6749; 1097-6825
• DOI: 10.1016/j.jaci.2017.06.037

Adult-onset severe asthma is characterized by highly symptomatic disease despite high-intensity asthma treatments. Understanding of the underlying pathways of this heterogeneous disease is needed for the development of targeted treatments. Gene set variation analysis is a statistical technique used to identify gene profiles in heterogeneous samples. We sought to identify gene profiles associated with adult-onset severe asthma. This was a cross-sectional, observational study in which adult patients with adult-onset of asthma (defined as starting at age ≥18 years) as compared with childhood-onset severe asthma (