Taxonomic assignment of the benthic toxigenic dinoflagellate Gambierdiscus sp. type 6 as Gambierdiscus balechii (Dinophyceae), including its distribution and ciguatoxicity

• Published in: Harmful algae 2017-07, Vol.67, p.107-118
• Main Authors: Dai, Xinfeng, Mak, Yim Ling, Lu, Chung-Kuang, Mei, Hua-Hsuan, Wu, Jia Jun, Lee, Wai Hin, Chan, Leo Lai, Lim, Po Teen, Mustapa, Nurin Izzati, Lim, Hong Chang, Wolf, Matthias, Li, Dongrong, Luo, Zhaohe, Gu, Haifeng, Leaw, Chui Pin, Lu, Douding
• Format: Article
• Language: English
• Subjects: Base Sequence; Benthic harmful algal bloom; Cell Size; Ciguatera fish poisoning; Ciguatoxins - toxicity; Dinoflagellida - classification; Dinoflagellida - cytology; Dinoflagellida - ultrastructure; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Kiribati; Likelihood Functions; LSU rDNA; Malaysia; Nucleic Acid Conformation; Phylogeny; Phylogeography; SSU rDNA
• ISSN: 1568-9883; 1878-1470
• DOI: 10.1016/j.hal.2017.07.002

•The taxonomic status of Gambierdiscus sp. type 6 is evaluated in this study.•The ribotype is now assigned as G. balechii based on morphological and molecular evidence.•The species is widely distributed in tropical Pacific coral reefs.•The central Pacific strains are ciguatoxic. Recent molecular phylogenetic studies of Gambierdiscus species flagged several new species and genotypes, thus leading to revitalizing its systematics. The inter-relationships of clades revealed by the primary sequence information of nuclear ribosomal genes (rDNA), however, can sometimes be equivocal, and therefore, in this study, the taxonomic status of a ribotype, Gambierdiscus sp. type 6, was evaluated using specimens collected from the original locality, Marakei Island, Republic of Kiribati; and specimens found in Rawa Island, Peninsular Malaysia, were further used for comparison. Morphologically, the ribotype cells resembled G. scabrosus, G. belizeanus, G. balechii, G. cheloniae and G. lapillus in thecal ornamentation, where the thecal surfaces are reticulate-foveated, but differed from G. scabrosus by its hatchet-shaped Plate 2′, and G. belizeanus by the asymmetrical Plate 3′. To identify the phylogenetic relationship of this ribotype, a large dataset of the large subunit (LSU) and small subunit (SSU) rDNAs were compiled, and performed comprehensive analyses, using Bayesian-inference, maximum-parsimony, and maximum-likelihood, for the latter two incorporating the sequence-structure information of the SSU rDNA. Both the LSU and SSU rDNA phylogenetic trees displayed an identical topology and supported the hypothesis that the relationship between Gambierdiscus sp. type 6 and G. balechii was monophyletic. As a result, the taxonomic status of Gambierdiscus sp. type 6 was revised, and assigned as Gambierdiscus balechii. Toxicity analysis using neuroblastoma N2A assay confirmed that the Central Pacific strains were toxic, ranging from 1.1 to 19.9 fg P-CTX-1 eq cell−1, but no toxicity was detected in a Western Pacific strain. This suggested that the species might be one of the species contributing to the high incidence rate of ciguatera fish poisoning in Marakei Island.