A novel type 1/2 hybrid IncC plasmid carrying fifteen antimicrobial resistance genes recovered from Proteus mirabilis in China

• Published in: Plasmid 2017-09, Vol.93, p.1-5
• Main Authors: Lei, Chang-Wei, Kong, Ling-Han, Ma, Su-Zhen, Liu, Bi-Hui, Chen, Yan-Peng, Zhang, An-Yun, Wang, Hong-Ning
• Format: Article
• Language: English
• Subjects: Animals; Bacterial Proteins - genetics; Chickens - microbiology; China; CMY-2; Drug Resistance, Multiple, Bacterial - genetics; Escherichia coli - genetics; Genes, Bacterial - genetics; Hybrid; IncC; Multidrug resistance; Phylogeny; Plasmid; Plasmids - genetics; Proteus mirabilis; Proteus mirabilis - genetics
• ISSN: 0147-619X; 1095-9890
• DOI: 10.1016/j.plasmid.2017.07.002

IncC plasmids are of great concern as vehicles of broad-spectrum cephalosporins and carbapenems resistance genes blaCMY and blaNDM. The aim of this study was to sequence and characterize a multidrug resistance (MDR) IncC plasmid (pPm14C18) recovered from Proteus mirabilis. pPm14C18 was identified in a CMY-2-producing P. mirabilis isolate from chicken in China in 2014, and could be transferred to Escherichia coli conferring an MDR phenotype. Whole genome sequencing confirmed pPm14C18 was a novel type 1/2 hybrid IncC plasmid 165,992bp in size, containing fifteen antimicrobial resistance genes. It harboured a novel MDR mosaic region comprised of a hybrid Tn21tnp-pDUmer, in which blaCTX-M-65, dfrA32 and ereA were firstly reported in IncC plasmid. Phylogenetic relationship reconstruction based on the nucleotide sequences of the 52 IncC backbones showed all type 1 IncC plasmids were clustered into one clade, and then merged with pPm14C18 and finally with the type 2 IncC plasmids and another type 1/2 hybrid IncC plasmid pYR1. The MDR IncC plasmids in P. mirabilis of animal origin might threaten public health, which should be drawn more attention. •The IncC plasmid pPm14C18 carrying blaCMY-2 gene recovered from Proteus mirabilis was completely sequenced.•It is a novel type 1/2 hybrid IncC plasmid 165,992bp in size harboring fifteen antimicrobial resistance genes.•It contains a novel 30.60-kb multidrug resistant mosaic region comprised of a hybrid Tn21tnp-pDUmer.•pPm14C18 can be easily transferred to Escherichia coli.•Phylogenetic relationship reconstruction reveals the evolution of the IncC backbones.