Loading…

Local anaesthetic refinement of pentobarbital euthanasia reduces abdominal writhing without affecting immunohistochemical endpoints in rats

Sodium pentobarbital is a commonly used agent for euthanizing laboratory rats, however its high pH can cause abdominal discomfort after intraperitoneal injection. Previous studies suggest that the addition of a local anaesthetic may alleviate this discomfort, but the practice has not been widely ado...

Full description

Saved in:
Bibliographic Details
Published in:Laboratory animals (London) 2018-04, Vol.52 (2), p.152-162
Main Authors: Khoo, Shaun Y-S, Lay, Belinda P P, Joya, Josephine, McNally, Gavan P
Format: Article
Language:English
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c379t-1ccd543450681c0197bfd1cbb890018da0e75c3073deb3e4670f50a5d9c797993
cites cdi_FETCH-LOGICAL-c379t-1ccd543450681c0197bfd1cbb890018da0e75c3073deb3e4670f50a5d9c797993
container_end_page 162
container_issue 2
container_start_page 152
container_title Laboratory animals (London)
container_volume 52
creator Khoo, Shaun Y-S
Lay, Belinda P P
Joya, Josephine
McNally, Gavan P
description Sodium pentobarbital is a commonly used agent for euthanizing laboratory rats, however its high pH can cause abdominal discomfort after intraperitoneal injection. Previous studies suggest that the addition of a local anaesthetic may alleviate this discomfort, but the practice has not been widely adopted. We examined the effect of combining lidocaine with pentobarbital on abdominal writhing, defecation, ultrasonic vocalizations, the rat grimace scale and immunohistochemical staining for c-Fos in the nucleus accumbens and basolateral amygdala of the brain. We also compared the amount of abdominal writhing following intraperitoneal administration of pentobarbital–lidocaine with that of pentobarbital–bupivacaine. Our results show that lidocaine reduces abdominal writhing and defecation without affecting immunohistochemistry for c-Fos or latency to loss of posture. However, scores on the rat grimace scale were low in both situations and almost no ultrasonic vocalizations were recorded. Additionally, we found that the amount of abdominal writhing was not significantly different when bupivacaine was used rather than lidocaine. Our results suggest that pentobarbital-induced euthanasia can be refined with the addition of lidocaine or other local anaesthetics.
doi_str_mv 10.1177/0023677217721260
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1924903993</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sage_id>10.1177_0023677217721260</sage_id><sourcerecordid>1924903993</sourcerecordid><originalsourceid>FETCH-LOGICAL-c379t-1ccd543450681c0197bfd1cbb890018da0e75c3073deb3e4670f50a5d9c797993</originalsourceid><addsrcrecordid>eNp1kU9PxCAQxYnRuOvq3ZPh6KUKpS3laIz_kk286LmhMN2y2cIKNBs_g19aml09mHggL_B-MxPeIHRJyQ2lnN8SkrOK85xOJ6_IEZpTXtYZTe4xmk92NvkzdBbCOl1pUZNTNMvrRJWsmKOvpVNyg6WVEGIP0SjsoTMWBrARuw5vk7pW-tbExMEY-8QGIxOmRwUBy1a7wdhk7ryJvbErvEvqxohl14GK04sZhtG63oToVA-DmWaC1VtnbAzYWOxlDOfopJObABcHXaD3x4e3--ds-fr0cn-3zBTjImZUKV0WrChJVVNFqOBtp6lq21oQQmstCfBSMcKZhpZBUXHSlUSWWiguuBBsga73fbfefYzp381ggoLNRlpwY2ioyAtBWCITSvao8i6ElEyz9WaQ_rOhpJlW0PxdQSq5OnQf2wH0b8FP5gnI9kCQK2jWbvQpvPB_w2_x0JGg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1924903993</pqid></control><display><type>article</type><title>Local anaesthetic refinement of pentobarbital euthanasia reduces abdominal writhing without affecting immunohistochemical endpoints in rats</title><source>Sage Journals Online</source><creator>Khoo, Shaun Y-S ; Lay, Belinda P P ; Joya, Josephine ; McNally, Gavan P</creator><creatorcontrib>Khoo, Shaun Y-S ; Lay, Belinda P P ; Joya, Josephine ; McNally, Gavan P</creatorcontrib><description>Sodium pentobarbital is a commonly used agent for euthanizing laboratory rats, however its high pH can cause abdominal discomfort after intraperitoneal injection. Previous studies suggest that the addition of a local anaesthetic may alleviate this discomfort, but the practice has not been widely adopted. We examined the effect of combining lidocaine with pentobarbital on abdominal writhing, defecation, ultrasonic vocalizations, the rat grimace scale and immunohistochemical staining for c-Fos in the nucleus accumbens and basolateral amygdala of the brain. We also compared the amount of abdominal writhing following intraperitoneal administration of pentobarbital–lidocaine with that of pentobarbital–bupivacaine. Our results show that lidocaine reduces abdominal writhing and defecation without affecting immunohistochemistry for c-Fos or latency to loss of posture. However, scores on the rat grimace scale were low in both situations and almost no ultrasonic vocalizations were recorded. Additionally, we found that the amount of abdominal writhing was not significantly different when bupivacaine was used rather than lidocaine. Our results suggest that pentobarbital-induced euthanasia can be refined with the addition of lidocaine or other local anaesthetics.</description><identifier>ISSN: 0023-6772</identifier><identifier>EISSN: 1758-1117</identifier><identifier>DOI: 10.1177/0023677217721260</identifier><identifier>PMID: 28758534</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><ispartof>Laboratory animals (London), 2018-04, Vol.52 (2), p.152-162</ispartof><rights>The Author(s) 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c379t-1ccd543450681c0197bfd1cbb890018da0e75c3073deb3e4670f50a5d9c797993</citedby><cites>FETCH-LOGICAL-c379t-1ccd543450681c0197bfd1cbb890018da0e75c3073deb3e4670f50a5d9c797993</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925,79364</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28758534$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Khoo, Shaun Y-S</creatorcontrib><creatorcontrib>Lay, Belinda P P</creatorcontrib><creatorcontrib>Joya, Josephine</creatorcontrib><creatorcontrib>McNally, Gavan P</creatorcontrib><title>Local anaesthetic refinement of pentobarbital euthanasia reduces abdominal writhing without affecting immunohistochemical endpoints in rats</title><title>Laboratory animals (London)</title><addtitle>Lab Anim</addtitle><description>Sodium pentobarbital is a commonly used agent for euthanizing laboratory rats, however its high pH can cause abdominal discomfort after intraperitoneal injection. Previous studies suggest that the addition of a local anaesthetic may alleviate this discomfort, but the practice has not been widely adopted. We examined the effect of combining lidocaine with pentobarbital on abdominal writhing, defecation, ultrasonic vocalizations, the rat grimace scale and immunohistochemical staining for c-Fos in the nucleus accumbens and basolateral amygdala of the brain. We also compared the amount of abdominal writhing following intraperitoneal administration of pentobarbital–lidocaine with that of pentobarbital–bupivacaine. Our results show that lidocaine reduces abdominal writhing and defecation without affecting immunohistochemistry for c-Fos or latency to loss of posture. However, scores on the rat grimace scale were low in both situations and almost no ultrasonic vocalizations were recorded. Additionally, we found that the amount of abdominal writhing was not significantly different when bupivacaine was used rather than lidocaine. Our results suggest that pentobarbital-induced euthanasia can be refined with the addition of lidocaine or other local anaesthetics.</description><issn>0023-6772</issn><issn>1758-1117</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp1kU9PxCAQxYnRuOvq3ZPh6KUKpS3laIz_kk286LmhMN2y2cIKNBs_g19aml09mHggL_B-MxPeIHRJyQ2lnN8SkrOK85xOJ6_IEZpTXtYZTe4xmk92NvkzdBbCOl1pUZNTNMvrRJWsmKOvpVNyg6WVEGIP0SjsoTMWBrARuw5vk7pW-tbExMEY-8QGIxOmRwUBy1a7wdhk7ryJvbErvEvqxohl14GK04sZhtG63oToVA-DmWaC1VtnbAzYWOxlDOfopJObABcHXaD3x4e3--ds-fr0cn-3zBTjImZUKV0WrChJVVNFqOBtp6lq21oQQmstCfBSMcKZhpZBUXHSlUSWWiguuBBsga73fbfefYzp381ggoLNRlpwY2ioyAtBWCITSvao8i6ElEyz9WaQ_rOhpJlW0PxdQSq5OnQf2wH0b8FP5gnI9kCQK2jWbvQpvPB_w2_x0JGg</recordid><startdate>201804</startdate><enddate>201804</enddate><creator>Khoo, Shaun Y-S</creator><creator>Lay, Belinda P P</creator><creator>Joya, Josephine</creator><creator>McNally, Gavan P</creator><general>SAGE Publications</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201804</creationdate><title>Local anaesthetic refinement of pentobarbital euthanasia reduces abdominal writhing without affecting immunohistochemical endpoints in rats</title><author>Khoo, Shaun Y-S ; Lay, Belinda P P ; Joya, Josephine ; McNally, Gavan P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c379t-1ccd543450681c0197bfd1cbb890018da0e75c3073deb3e4670f50a5d9c797993</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Khoo, Shaun Y-S</creatorcontrib><creatorcontrib>Lay, Belinda P P</creatorcontrib><creatorcontrib>Joya, Josephine</creatorcontrib><creatorcontrib>McNally, Gavan P</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Laboratory animals (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Khoo, Shaun Y-S</au><au>Lay, Belinda P P</au><au>Joya, Josephine</au><au>McNally, Gavan P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Local anaesthetic refinement of pentobarbital euthanasia reduces abdominal writhing without affecting immunohistochemical endpoints in rats</atitle><jtitle>Laboratory animals (London)</jtitle><addtitle>Lab Anim</addtitle><date>2018-04</date><risdate>2018</risdate><volume>52</volume><issue>2</issue><spage>152</spage><epage>162</epage><pages>152-162</pages><issn>0023-6772</issn><eissn>1758-1117</eissn><abstract>Sodium pentobarbital is a commonly used agent for euthanizing laboratory rats, however its high pH can cause abdominal discomfort after intraperitoneal injection. Previous studies suggest that the addition of a local anaesthetic may alleviate this discomfort, but the practice has not been widely adopted. We examined the effect of combining lidocaine with pentobarbital on abdominal writhing, defecation, ultrasonic vocalizations, the rat grimace scale and immunohistochemical staining for c-Fos in the nucleus accumbens and basolateral amygdala of the brain. We also compared the amount of abdominal writhing following intraperitoneal administration of pentobarbital–lidocaine with that of pentobarbital–bupivacaine. Our results show that lidocaine reduces abdominal writhing and defecation without affecting immunohistochemistry for c-Fos or latency to loss of posture. However, scores on the rat grimace scale were low in both situations and almost no ultrasonic vocalizations were recorded. Additionally, we found that the amount of abdominal writhing was not significantly different when bupivacaine was used rather than lidocaine. Our results suggest that pentobarbital-induced euthanasia can be refined with the addition of lidocaine or other local anaesthetics.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>28758534</pmid><doi>10.1177/0023677217721260</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0023-6772
ispartof Laboratory animals (London), 2018-04, Vol.52 (2), p.152-162
issn 0023-6772
1758-1117
language eng
recordid cdi_proquest_miscellaneous_1924903993
source Sage Journals Online
title Local anaesthetic refinement of pentobarbital euthanasia reduces abdominal writhing without affecting immunohistochemical endpoints in rats
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T06%3A58%3A09IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Local%20anaesthetic%20refinement%20of%20pentobarbital%20euthanasia%20reduces%20abdominal%20writhing%20without%20affecting%20immunohistochemical%20endpoints%20in%20rats&rft.jtitle=Laboratory%20animals%20(London)&rft.au=Khoo,%20Shaun%20Y-S&rft.date=2018-04&rft.volume=52&rft.issue=2&rft.spage=152&rft.epage=162&rft.pages=152-162&rft.issn=0023-6772&rft.eissn=1758-1117&rft_id=info:doi/10.1177/0023677217721260&rft_dat=%3Cproquest_cross%3E1924903993%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c379t-1ccd543450681c0197bfd1cbb890018da0e75c3073deb3e4670f50a5d9c797993%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1924903993&rft_id=info:pmid/28758534&rft_sage_id=10.1177_0023677217721260&rfr_iscdi=true