Extracorporeal shock wave treatment can selectively destroy end plates in neuromuscular junctions

ABSTRACT Introduction This study assesses the effect of radial extracorporeal shock wave (rESW) exposure on neuromuscular transmission and neuromuscular junction (NMJ) morphology. Methods We applied 2,000 rESWs at 0.18 mJ/mm2 and a frequency of 15 Hz to the right calf of male rats, measured the comp...

Full description

Saved in:
Bibliographic Details
Published in:Muscle & nerve 2018-03, Vol.57 (3), p.466-472
Main Authors: Kenmoku, Tomonori, Nemoto, Noriko, Iwakura, Nahoko, Ochiai, Nobuyasu, Uchida, Kentaro, Saisu, Takashi, Ohtori, Seiji, Nakagawa, Koichi, Sasho, Takahisa, Takaso, Masashi
Format: Article
Language:English
Subjects:
acetylcholine receptor
Acetylcholine receptors
Acoustic impedance
Action potential
Action Potentials - physiology
Animals
Dystonia
Electron microscopy
end plate
End plates
Exposure
extracorporeal shock wave therapy
Extracorporeal Shockwave Therapy
Latency
Male
Microscopy, Electron
Morphology
Motor Endplate - physiology
Motor Endplate - ultrastructure
Muscle, Skeletal - innervation
Muscle, Skeletal - ultrastructure
Muscles
neuromuscular junction
Neuromuscular Junction - physiology
Neuromuscular Junction - ultrastructure
Neuromuscular junctions
Neuromuscular transmission
Plates
Presynapse
Rats
Rats, Sprague-Dawley
Receptors
Rodents
Spasticity
synaptic cleft
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:ABSTRACT Introduction This study assesses the effect of radial extracorporeal shock wave (rESW) exposure on neuromuscular transmission and neuromuscular junction (NMJ) morphology. Methods We applied 2,000 rESWs at 0.18 mJ/mm2 and a frequency of 15 Hz to the right calf of male rats, measured the compound muscle action potential (CMAP), and examined NMJ morphology using electron microscopy. Left calf muscles were used as controls. Results rESW exposure significantly reduced CMAP amplitude without delayed latency in exposed muscles compared with controls. All rESW‐exposed muscles exhibited NMJs with irregular end plates. Mean interjunctional fold interval was significantly increased compared with controls. However, axon terminals and muscle fibers surrounding NMJs with irregular end plates were unchanged. Discussion This localized destruction of end plates may be caused by differences in acoustic impedance induced by the density of acetylcholine receptors. These results provide a possible mechanism for the effectiveness of rESW treatment for spasticity and dystonia. Muscle Nerve 57: 466–472, 2018
ISSN:0148-639X
1097-4598
DOI:10.1002/mus.25754