N1, N14-diferuloylspermine as an antioxidative phytochemical contained in leaves of Cardamine fauriei

• Published in: Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2017-10, Vol.81 (10), p.1855-1860
• Main Authors: Abe, Keima, Matsuura, Hideyuki, Ukai, Mitsuko, Shimura, Hanako, Koshino, Hiroyuki, Suzuki, Takashi
• Format: Article
• Language: English
• Subjects: Brassicaceae; ESR spin trapping; ORAC; polyamine feruloyl amide; stereoisomer
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1080/09168451.2017.1356214

Most Brassicaceae vegetables are ideal dietary sources of antioxidants beneficial for human health. Cardamine fauriei (Ezo-wasabi in Japanese) is a wild, edible Brassicaceae herb native to Hokkaido, Japan. To clarify the main antioxidative phytochemical, an 80% methanol extraction from the leaves was fractionated with Diaion® HP-20, Sephadex® LH-20, and Sep-Pak® C18 cartridges, and the fraction with strong antioxidant activity depending on DPPH method was purified by HPLC. Based on the analyses using HRESIMS and MS/MS, the compound might be N 1 , N 14 -diferuloylspermine. This rare phenol compound was chemically synthesized, whose data on HPLC, MS and 1 H NMR were compared with those of naturally derived compound from C. fauriei. All results indicated they were the same compound. The radical-scavenging properties of diferuloylspermine were evaluated by ORAC and ESR spin trapping methods, with the diferuloylspermine showing high scavenging activities of the ROO · , O 2 ·− , and HO · radicals as was those of conventional antioxidants.