Rapid Characterization of Constituents in Tribulus terrestris from Different Habitats by UHPLC/Q-TOF MS

A strategy for rapid identification of the chemical constituents from crude extracts of Tribulus terrestris was proposed using an informatics platform for the UHPLC/Q-TOF MS E data analyses. This strategy mainly utilizes neutral losses, characteristic fragments, and in-house library to rapidly ident...

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Bibliographic Details
Published in:Journal of the American Society for Mass Spectrometry 2017-11, Vol.28 (11), p.2302-2318
Main Authors: Zheng, Wei, Wang, Fangxu, Zhao, Yang, Sun, Xinguang, Kang, Liping, Fan, Ziquan, Qiao, Lirui, Yan, Renyi, Liu, Shuchen, Ma, Baiping
Format: Article
Language:English
Subjects:
Analytical Chemistry
Bioinformatics
Biotechnology
Chemistry
Chemistry and Materials Science
Chromatography, High Pressure Liquid - methods
Constituents
Flavonoids
Flavonoids - analysis
Flavonoids - chemistry
Fragments
Informatics
Isomers
Libraries
Mass spectrometry
Mass Spectrometry - methods
Organic Chemistry
Plant Extracts - chemistry
Proteomics
Research Article
Saponins
Saponins - analysis
Saponins - chemistry
Strategy
Traditional Chinese medicine
Tribulus - chemistry
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Summary:A strategy for rapid identification of the chemical constituents from crude extracts of Tribulus terrestris was proposed using an informatics platform for the UHPLC/Q-TOF MS E data analyses. This strategy mainly utilizes neutral losses, characteristic fragments, and in-house library to rapidly identify the structure of the compounds. With this strategy, rapid characterization of the chemical components of T. terrestris from Beijing, China was successfully achieved. A total of 82 steroidal saponins and nine flavonoids were identified or tentatively identified from T. terrestris . Among them, 15 new components were deduced based on retention times and characteristic MS fragmentation patterns. Furthermore, the chemical components of T. terrestris , including the other two samples from Xinjiang Uygur Autonomous region, China, and Rome, Italy, were also identified with this strategy. Altogether, 141 chemical components were identified from these three samples, of which 39 components were identified or tentatively identified as new compounds, including 35 groups of isomers. It demonstrated that this strategy provided an efficient protocol for the rapid identification of chemical constituents in complex samples such as traditional Chinese medicines (TCMs) by UHPLC/Q-TOF MS E with informatics platform. Graphical Abstract ᅟ
ISSN:1044-0305
1879-1123
DOI:10.1007/s13361-017-1761-5