3M Petrifilm Enterobacteriaceae count Plate method for enumeration of Enterobacteriaceae in selected foods: Collaborative study

The practice of detecting and enumerating all oxidase-negative, glucose-fermenting-Gram-negative rods (i.e., the family Enterobacteriaceae) is used to indicate unsanitary or inadequate food processing conditions. The objective of this interlaboratory collaborative study was to evaluate and compare t...

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Bibliographic Details
Published in:Journal of AOAC International 2003-07, Vol.86 (4), p.802-814
Main Authors: SILBERNAGEL, Karen M, LINDBERG, Kathryn G
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Brassica - microbiology
Cheese - microbiology
Colony Count, Microbial - methods
Enterobacteriaceae
Enterobacteriaceae - isolation & purification
Flour - microbiology
Food industries
Food Microbiology
Frozen Foods - microbiology
Fundamental and applied biological sciences. Psychology
General aspects
Indicators and Reagents
Laboratories
Methods of analysis, processing and quality control, regulation, standards
Milk - microbiology
Nuts - microbiology
Reproducibility of Results
Sensitivity and Specificity
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Summary:The practice of detecting and enumerating all oxidase-negative, glucose-fermenting-Gram-negative rods (i.e., the family Enterobacteriaceae) is used to indicate unsanitary or inadequate food processing conditions. The objective of this interlaboratory collaborative study was to evaluate and compare the methods described in Standard Methods for the Examination of Dairy Products (SMEDP) and the Compendium of Methods for the Microbiological Examination of Foods (Compendium) with a commercial product, the 3M Petrifilm Enterobacteriaceae Count Plate, for the recovery of Enterobacteriaceae in foods. Six foods--cheddar cheese, milk, flour, frozen prepared meals, frozen broccoli, and nut pieces--were analyzed for Enterobacteriaceae by 12 collaborating laboratories. For each food tested, the collaborators received 8 blind test portions consisting of a control test portion and 3 levels of inoculated test portion, each in duplicate. Each test portion was tested by the Petrifilm Enterobacteriaceae Count Plate method as well as the SMEDP or Compendium methods. The precision estimates (repeatability or within-laboratory variation, and reproducibility or between-laboratory variation) were calculated with standard statistical techniques.
ISSN:1060-3271
1944-7922
DOI:10.1093/jaoac/86.4.802