Understanding and improving aggregated gold nanoparticle/dsDNA interactions by molecular spectroscopy and deconvolution methods

It is well known that single-stranded DNA (ssDNA) is easily able to adsorb on citrate-capped, non-functionalized gold nanoparticles (AuNPs). However, the affinity of double-stranded DNA (dsDNA) for them is much more limited. The present work demonstrates that long dsDNA suffers from a bending confor...

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Bibliographic Details
Published in:Physical chemistry chemical physics : PCCP 2017, Vol.19 (24), p.16113-16123
Main Authors: Carnerero, Jose M, Jimenez-Ruiz, Aila, Grueso, Elia M, Prado-Gotor, Rafael
Format: Article
Language:English
Subjects:
Agglomeration
Animals
Atomic force microscopy
Bending
Cattle
Circular Dichroism
Deconvolution
Deoxyribonucleic acid
DNA - analysis
DNA - chemistry
DNA, Single-Stranded - chemistry
Dynamic Light Scattering
Electrolytes - chemistry
Gold
Gold - chemistry
Kinetics
Metal Nanoparticles - chemistry
Microscopy, Atomic Force
Nanoparticles
Nanostructure
Spectrophotometry
Viscosity
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Summary:It is well known that single-stranded DNA (ssDNA) is easily able to adsorb on citrate-capped, non-functionalized gold nanoparticles (AuNPs). However, the affinity of double-stranded DNA (dsDNA) for them is much more limited. The present work demonstrates that long dsDNA suffers from a bending conformational change when anionic nanoparticles are present in solution. A striking decrease in the persistence length of the double helix in the absence of salt is observed through dynamic light scattering (DLS), viscometric, and atomic force microscopy (AFM) methods. Long dsDNA is therefore shown to be able to interact with anionic gold nanoparticles. To date, only ssDNA detection has been described by making use of interparticle cross-linking aggregation mechanisms; however, the data shown in this work allow for the development of new methods for detecting dsDNA in solution by using aggregated AuNPs as a starting point. The aggregation state is induced by the controlled addition of an inert electrolyte. A deconvolution procedure of the experimental plasmon shows how individual bands corresponding to aggregated nanoclusters diminish as the DNA concentration increases in the presence of 0.075 M NaCl.
ISSN:1463-9076
1463-9084
DOI:10.1039/c7cp02219k