Combined cross-diffusion microbatch method and seeding technique to enhance protein crystallization based on a common dispersing agent

We presented a systematic method that combined the cross-diffusion microbatch method (CDM) with the seeding technique to enhance protein crystal reproducibility based on a "common dispersing agent". The common dispersing agent is used to dilute the crystal seeds. Seven proteins were screen...

Full description

Saved in:
Bibliographic Details
Published in:CrystEngComm 2017, Vol.19 (24), p.3237-3243
Main Authors: Hou, Hai, Wang, Bo, Xie, Peng-Peng, Guo, Yun-Zhu, Li, Jin, Yin, Da-Chuan
Format: Article
Language:English
Subjects:
Crystallization
Crystals
Dispersion
Nucleation
Proteins
Reproducibility
Seeds
X ray diffraction
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c264t-9f8a3c3b60b1f1e974320ca8f25adad2eba781517a0637e8e2f13b633de939b73
cites cdi_FETCH-LOGICAL-c264t-9f8a3c3b60b1f1e974320ca8f25adad2eba781517a0637e8e2f13b633de939b73
container_end_page 3243
container_issue 24
container_start_page 3237
container_title CrystEngComm
container_volume 19
creator Hou, Hai
Wang, Bo
Xie, Peng-Peng
Guo, Yun-Zhu
Li, Jin
Yin, Da-Chuan
description We presented a systematic method that combined the cross-diffusion microbatch method (CDM) with the seeding technique to enhance protein crystal reproducibility based on a "common dispersing agent". The common dispersing agent is used to dilute the crystal seeds. Seven proteins were screened, and more crystallization conditions were found. X-ray diffraction showed that the combined method is practical and useful for obtaining high-quality protein crystals.
doi_str_mv 10.1039/C7CE00664K
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1925879089</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1925879089</sourcerecordid><originalsourceid>FETCH-LOGICAL-c264t-9f8a3c3b60b1f1e974320ca8f25adad2eba781517a0637e8e2f13b633de939b73</originalsourceid><addsrcrecordid>eNpNkM1OwzAQhC0EEqVw4Ql8REgBO06T-Iii8iMqcYFztLHXjVFil9g9lAfguXEpEpx2tBp9MxpCLjm74UzI26ZqloyVZfF8RGa8KMusZkIc_9On5CyEd8Z4wTmbka_Gj511qKmafAiZtsZsg_WOjjZ9OoiqpyPG3msKTtOAqK1b04iqd_ZjizR6iq4Hp5BuJh_RuoTahQjDYD8h7lEdhBSQBFDlxzEJbcMGp7AnwRpdPCcnBoaAF793Tt7ul6_NY7Z6eXhq7laZyssiZtLUIJToStZxw1FWhciZgtrkC9Cgc-ygqvmCV8BKUWGNueHJLYRGKWRXiTm5OnBT1VQ-xHa0QeEwgEO_DS2X-aKuJKtlsl4frD_DTGjazWRHmHYtZ-1-7PZvbPENxwp0zQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1925879089</pqid></control><display><type>article</type><title>Combined cross-diffusion microbatch method and seeding technique to enhance protein crystallization based on a common dispersing agent</title><source>Royal Society of Chemistry:Jisc Collections:Royal Society of Chemistry Read and Publish 2022-2024 (reading list)</source><creator>Hou, Hai ; Wang, Bo ; Xie, Peng-Peng ; Guo, Yun-Zhu ; Li, Jin ; Yin, Da-Chuan</creator><creatorcontrib>Hou, Hai ; Wang, Bo ; Xie, Peng-Peng ; Guo, Yun-Zhu ; Li, Jin ; Yin, Da-Chuan</creatorcontrib><description>We presented a systematic method that combined the cross-diffusion microbatch method (CDM) with the seeding technique to enhance protein crystal reproducibility based on a "common dispersing agent". The common dispersing agent is used to dilute the crystal seeds. Seven proteins were screened, and more crystallization conditions were found. X-ray diffraction showed that the combined method is practical and useful for obtaining high-quality protein crystals.</description><identifier>ISSN: 1466-8033</identifier><identifier>EISSN: 1466-8033</identifier><identifier>DOI: 10.1039/C7CE00664K</identifier><language>eng</language><subject>Crystallization ; Crystals ; Dispersion ; Nucleation ; Proteins ; Reproducibility ; Seeds ; X ray diffraction</subject><ispartof>CrystEngComm, 2017, Vol.19 (24), p.3237-3243</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c264t-9f8a3c3b60b1f1e974320ca8f25adad2eba781517a0637e8e2f13b633de939b73</citedby><cites>FETCH-LOGICAL-c264t-9f8a3c3b60b1f1e974320ca8f25adad2eba781517a0637e8e2f13b633de939b73</cites><orcidid>0000-0003-2161-5867 ; 0000-0003-4199-354X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids></links><search><creatorcontrib>Hou, Hai</creatorcontrib><creatorcontrib>Wang, Bo</creatorcontrib><creatorcontrib>Xie, Peng-Peng</creatorcontrib><creatorcontrib>Guo, Yun-Zhu</creatorcontrib><creatorcontrib>Li, Jin</creatorcontrib><creatorcontrib>Yin, Da-Chuan</creatorcontrib><title>Combined cross-diffusion microbatch method and seeding technique to enhance protein crystallization based on a common dispersing agent</title><title>CrystEngComm</title><description>We presented a systematic method that combined the cross-diffusion microbatch method (CDM) with the seeding technique to enhance protein crystal reproducibility based on a "common dispersing agent". The common dispersing agent is used to dilute the crystal seeds. Seven proteins were screened, and more crystallization conditions were found. X-ray diffraction showed that the combined method is practical and useful for obtaining high-quality protein crystals.</description><subject>Crystallization</subject><subject>Crystals</subject><subject>Dispersion</subject><subject>Nucleation</subject><subject>Proteins</subject><subject>Reproducibility</subject><subject>Seeds</subject><subject>X ray diffraction</subject><issn>1466-8033</issn><issn>1466-8033</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNpNkM1OwzAQhC0EEqVw4Ql8REgBO06T-Iii8iMqcYFztLHXjVFil9g9lAfguXEpEpx2tBp9MxpCLjm74UzI26ZqloyVZfF8RGa8KMusZkIc_9On5CyEd8Z4wTmbka_Gj511qKmafAiZtsZsg_WOjjZ9OoiqpyPG3msKTtOAqK1b04iqd_ZjizR6iq4Hp5BuJh_RuoTahQjDYD8h7lEdhBSQBFDlxzEJbcMGp7AnwRpdPCcnBoaAF793Tt7ul6_NY7Z6eXhq7laZyssiZtLUIJToStZxw1FWhciZgtrkC9Cgc-ygqvmCV8BKUWGNueHJLYRGKWRXiTm5OnBT1VQ-xHa0QeEwgEO_DS2X-aKuJKtlsl4frD_DTGjazWRHmHYtZ-1-7PZvbPENxwp0zQ</recordid><startdate>2017</startdate><enddate>2017</enddate><creator>Hou, Hai</creator><creator>Wang, Bo</creator><creator>Xie, Peng-Peng</creator><creator>Guo, Yun-Zhu</creator><creator>Li, Jin</creator><creator>Yin, Da-Chuan</creator><scope>AAYXX</scope><scope>CITATION</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><orcidid>https://orcid.org/0000-0003-2161-5867</orcidid><orcidid>https://orcid.org/0000-0003-4199-354X</orcidid></search><sort><creationdate>2017</creationdate><title>Combined cross-diffusion microbatch method and seeding technique to enhance protein crystallization based on a common dispersing agent</title><author>Hou, Hai ; Wang, Bo ; Xie, Peng-Peng ; Guo, Yun-Zhu ; Li, Jin ; Yin, Da-Chuan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c264t-9f8a3c3b60b1f1e974320ca8f25adad2eba781517a0637e8e2f13b633de939b73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Crystallization</topic><topic>Crystals</topic><topic>Dispersion</topic><topic>Nucleation</topic><topic>Proteins</topic><topic>Reproducibility</topic><topic>Seeds</topic><topic>X ray diffraction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hou, Hai</creatorcontrib><creatorcontrib>Wang, Bo</creatorcontrib><creatorcontrib>Xie, Peng-Peng</creatorcontrib><creatorcontrib>Guo, Yun-Zhu</creatorcontrib><creatorcontrib>Li, Jin</creatorcontrib><creatorcontrib>Yin, Da-Chuan</creatorcontrib><collection>CrossRef</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>CrystEngComm</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hou, Hai</au><au>Wang, Bo</au><au>Xie, Peng-Peng</au><au>Guo, Yun-Zhu</au><au>Li, Jin</au><au>Yin, Da-Chuan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Combined cross-diffusion microbatch method and seeding technique to enhance protein crystallization based on a common dispersing agent</atitle><jtitle>CrystEngComm</jtitle><date>2017</date><risdate>2017</risdate><volume>19</volume><issue>24</issue><spage>3237</spage><epage>3243</epage><pages>3237-3243</pages><issn>1466-8033</issn><eissn>1466-8033</eissn><abstract>We presented a systematic method that combined the cross-diffusion microbatch method (CDM) with the seeding technique to enhance protein crystal reproducibility based on a "common dispersing agent". The common dispersing agent is used to dilute the crystal seeds. Seven proteins were screened, and more crystallization conditions were found. X-ray diffraction showed that the combined method is practical and useful for obtaining high-quality protein crystals.</abstract><doi>10.1039/C7CE00664K</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0003-2161-5867</orcidid><orcidid>https://orcid.org/0000-0003-4199-354X</orcidid></addata></record>
fulltext fulltext
identifier ISSN: 1466-8033
ispartof CrystEngComm, 2017, Vol.19 (24), p.3237-3243
issn 1466-8033
1466-8033
language eng
recordid cdi_proquest_miscellaneous_1925879089
source Royal Society of Chemistry:Jisc Collections:Royal Society of Chemistry Read and Publish 2022-2024 (reading list)
subjects Crystallization
Crystals
Dispersion
Nucleation
Proteins
Reproducibility
Seeds
X ray diffraction
title Combined cross-diffusion microbatch method and seeding technique to enhance protein crystallization based on a common dispersing agent
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-02T04%3A26%3A36IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Combined%20cross-diffusion%20microbatch%20method%20and%20seeding%20technique%20to%20enhance%20protein%20crystallization%20based%20on%20a%20common%20dispersing%20agent&rft.jtitle=CrystEngComm&rft.au=Hou,%20Hai&rft.date=2017&rft.volume=19&rft.issue=24&rft.spage=3237&rft.epage=3243&rft.pages=3237-3243&rft.issn=1466-8033&rft.eissn=1466-8033&rft_id=info:doi/10.1039/C7CE00664K&rft_dat=%3Cproquest_cross%3E1925879089%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c264t-9f8a3c3b60b1f1e974320ca8f25adad2eba781517a0637e8e2f13b633de939b73%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1925879089&rft_id=info:pmid/&rfr_iscdi=true