Identification of actin as a direct proteomic target of berberine using an affinity-based chemical probe and elucidation of its modulatory role in actin assembly

Despite the diverse pharmacological activities of berberine, including anti-cancer and anti-inflammatory effects, the direct proteomic targets of berberine have remained largely unknown. Here, we have identified actin as a direct proteomic target of berberine using an affinity-based chemical probe....

Full description

Saved in:
Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2017-06, Vol.53 (52), p.7045-7047
Main Authors: Yi, Chae-Min, Yu, Jihyun, Kim, Hyunbin, Lee, Na-Rae, Kim, Sang Won, Lee, Noh-Jin, Lee, Jun, Seong, Jihye, Kim, Nam-Jung, Inn, Kyung-Soo
Format: Article
Language:English
Subjects:
Actins - biosynthesis
Actins - chemistry
Actins - metabolism
Anticancer properties
Assembly
Berberine - chemistry
Berberine - pharmacology
Biochemistry
In vitro testing
Molecular Probes - chemistry
Molecular Probes - pharmacology
Molecular Structure
Pharmacology
Proteomics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Despite the diverse pharmacological activities of berberine, including anti-cancer and anti-inflammatory effects, the direct proteomic targets of berberine have remained largely unknown. Here, we have identified actin as a direct proteomic target of berberine using an affinity-based chemical probe. In addition, we found that actin assembly was significantly modulated by berberine in vitro at the biochemical level and cellular level.
ISSN:1359-7345
1364-548X
DOI:10.1039/c7cc02789c