Prevalence of anti-DFS70 antibodies in patients with and without systemic autoimmune rheumatic diseases

Autoantibodies to the dense fine speckled 70 (DFS70) antigen are common among antinuclear antibodies (ANA) positive healthy individuals (HI). We assessed the prevalence of anti-DFS70 antibodies in patients with and without ANA-associated rheumatic diseases (AARDs) by two methods: chemiluminescent im...

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Bibliographic Details
Published in:Clinical and experimental rheumatology 2018-01, Vol.36 (1), p.121-126
Main Authors: Shovman, Ora, Gilburd, Boris, Chayat, Chen, Amital, Howard, Langevitz, Pnina, Watad, Abdulla, Guy, Adi, Perez, Dolores, Azoulay, Danielle, Blank, Miri, Segal, Yael, Bentow, Chelsea, Mahler, Michael, Shoenfeld, Yehuda
Format: Article
Language:English
Subjects:
Adaptor Proteins, Signal Transducing - immunology
Antibodies, Antinuclear - blood
Autoimmunity
Biomarkers - blood
Case-Control Studies
Fluorescent Antibody Technique, Indirect
Humans
Immunoassay
Immunosorbent Techniques
Luminescent Measurements
Predictive Value of Tests
Reproducibility of Results
Rheumatic Diseases - blood
Rheumatic Diseases - diagnosis
Rheumatic Diseases - epidemiology
Rheumatic Diseases - immunology
Seroepidemiologic Studies
Transcription Factors - immunology
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Summary:Autoantibodies to the dense fine speckled 70 (DFS70) antigen are common among antinuclear antibodies (ANA) positive healthy individuals (HI). We assessed the prevalence of anti-DFS70 antibodies in patients with and without ANA-associated rheumatic diseases (AARDs) by two methods: chemiluminescent immunoassay (CIA) and an indirect immunofluorescence (IIF) assay based on immunoadsorption for DFS70. Fifty-one ANA-positive sera samples from patients with confirmed clinical diagnosis of AARD, 92 samples from HI and 85 samples submitted to a reference laboratory for routine ANA testing were evaluated for the presence of anti-DFS70 antibodies. The samples were evaluated by QUANTA Flash DFS70 CIA using BIO-FLASH instrument and by NOVA Lite selected HEp-2 kit on NOVA View - an automated IIF system. Sera with DFS positive pattern were pre-absorbed with highly purified human DFS70 antigen, and then tested again. Twenty-four samples (10.5%) tested by QUANTA Flash DFS70 CIA were positive for anti-DFS70 antibodies. The prevalence of monospecific anti-DFS70 antibodies was significantly higher in healthy subjects than in patients with AARDs (10.9% vs. 1.9%, p=0.02). The frequency of anti-DFS70 antibodies in samples submitted for routine ANA testing was 15.2%. A very good agreement was found between CIA and the DFS pattern identified by the automated HEp-2 IIF (kappa=0.97). In 80% of the samples obtained from patients without AARDs, immunoadsorption effectively inhibited the anti-DFS70 antibodies. The data confirm that mono-specific anti-DFS70 antibodies are a strong discriminator between ANA positive HI and AARD patients, and their evaluation should be included in ANA testing algorithms.
ISSN:0392-856X