Continuous-release or burst-release of the antimicrobial peptide human lactoferrin 1-11 (hLF1-11) from calcium phosphate bone substitutes

Objectives: In order to identify possible drug delivery systems against resistant bone infection, we determined the release of the antimicrobial peptide (AMP) human lactoferrin 1-11 (hLF1-11) from commercially available bone substitutes. Methods: We combined six calcium phosphate cements and six gra...

Full description

Saved in:
Bibliographic Details
Published in:Journal of antimicrobial chemotherapy 2003-11, Vol.52 (5), p.853-855
Main Authors: Stallmann, Hein P., Faber, Christopher, Slotema, Eveline T., Lyaruu, D. M., Bronckers, Antonius L. J. J., Amerongen, Arie V. Nieuw, Wuisman, Paul I. J. M.
Format: Article
Language:English
Subjects:
Antibacterial agents
Antibiotics. Antiinfectious agents. Antiparasitic agents
biodegradable
Biodegradation, Environmental
Biological and medical sciences
bone infections
Bone Substitutes - chemistry
Calcium Phosphates - chemistry
carriers
Drug Delivery Systems - methods
human lactoferrin
Humans
Lactoferrin
Mass Spectrometry
Medical sciences
Methicillin Resistance
Peptide Fragments - chemistry
Peptide Fragments - pharmacology
Pharmacology. Drug treatments
Staphylococcus aureus
Staphylococcus aureus - drug effects
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Objectives: In order to identify possible drug delivery systems against resistant bone infection, we determined the release of the antimicrobial peptide (AMP) human lactoferrin 1-11 (hLF1-11) from commercially available bone substitutes. Methods: We combined six calcium phosphate cements and six granule-types with 5 mg/g hLF1-11 and measured its availability and release in vitro from cements (7 days) and granules (3 days). The integrity and antimicrobial activity of the hLF1-11 that was released during the first 24 h were measured, using mass spectrometry, and a killing assay on methicillin-resistant Staphylococcus aureus (MRSA). Results: Most of the cements showed burst release followed by low-level continuous release, whereas the coated granules showed high burst release for 24 h. After release the peptide was active (in nine of 12 materials) and intact. Conclusions: Different release profiles may be obtained by choosing the appropriate carrier, which supports the feasibility of biodegradable carriers releasing AMPs against resistant infections.
ISSN:0305-7453
1460-2091
1460-2091
DOI:10.1093/jac/dkg443