Ovarian cystocytes can repopulate the embryonic germ line and produce functional gametes

Ovarian tumors are formed either in the absence of Bam (bag-of-marbles) in germ-line cells or the overexpression of Dpp (decapentaplegic) in ovarian somatic cells. These tumor cells contain spectrosomes characteristic of ovarian germ-line stem cells and the immediate descendents called cystoblasts....

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2003-11, Vol.100 (24), p.14042-14045
Main Authors: Niki, Y, Mahowald, A.P
Format: Article
Language:English
Subjects:
Animals
Animals, Genetically Modified
bag-of-marbles protein
Biological Sciences
Biology
Cell transplantation
cystocytes
Drosophila - cytology
Drosophila - embryology
Drosophila - genetics
Drosophila melanogaster
Drosophila Proteins - genetics
embryo (animal)
Embryonic stem cells
Embryos
Female
gametogenesis
Gene Targeting
Genes, Insect
Germ cells
Gonads
heat stress
Lac Operon
oocytes
Oocytes - cytology
Ovarian cancer
ovarian neoplasms
Ovaries
Ovary - cytology
Ovary - embryology
Stem cell transplantation
Stem cells
Tumor stem cells
Tumors
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Summary:Ovarian tumors are formed either in the absence of Bam (bag-of-marbles) in germ-line cells or the overexpression of Dpp (decapentaplegic) in ovarian somatic cells. These tumor cells contain spectrosomes characteristic of ovarian germ-line stem cells and the immediate descendents called cystoblasts. We show that pole cells can successfully populate the gonad after transplantation to the dorsal mesoderm of host embryos following germ-band extension. By using this approach, we demonstrate that bam-cells can populate the gonad and become established as germ-line stem cells. Tumor cells containing the wild-type bam gene under heat shock transcriptional control are able to produce functional oocytes. Thus, stem cells/cystoblasts of the adult ovary are capable of forming stem cells in the embryonic ovary and recapitulating the development of the female germ line.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.2235591100