Synthesis of supermacroporous cryogel for bioreactors continuous starch hydrolysis

A bioreactor was built by means of immobilizing alpha‐amylase from Aspergillus oryzae by encapsulation, through cryopolymerization of acrylamide monomers for the continuous starch hydrolysis. The starch hydrolysis was evaluated regarding pH, the concentration of immobilized amylase on cryogel, the c...

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Bibliographic Details
Published in:Electrophoresis 2017-11, Vol.38 (22-23), p.2940-2946
Main Authors: Guilherme, Ederson Paulo Xavier, Oliveira, Jocilane Pereira, Carvalho, Lorendane Millena, Brandi, Igor Viana, Santos, Sérgio Henrique Sousa, Carvalho, Gleidson Giordano Pinto, Cota, Junio, Mara Aparecida de Carvalho, Bruna
Format: Article
Language:English
Subjects:
Acrylamide
Amylase
Amylases
Bioreactors
Conversion ratio
Cryogel
Enzymes
Hydrolysis
Maltose
Monolith
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Summary:A bioreactor was built by means of immobilizing alpha‐amylase from Aspergillus oryzae by encapsulation, through cryopolymerization of acrylamide monomers for the continuous starch hydrolysis. The starch hydrolysis was evaluated regarding pH, the concentration of immobilized amylase on cryogel, the concentration of starch solution and temperature. The maximum value for starch hydrolysis was achieved at pH 5.0, concentration of immobilized enzyme 111.44 mg amylase/gcryogel, concentration of starch solution 45 g/L and temperature of 35°C. The immobilized enzyme showed a conversion ratio ranging from 68.2 to 97.37%, depending on the pH and temperature employed. Thus, our results suggest that the alpha‐amylase from A. oryzae immobilized on cryogel monoliths represents a potential process for industrial production of maltose from starch hydrolysis.
ISSN:0173-0835
1522-2683
DOI:10.1002/elps.201700208