Nerve growth factor from seminal plasma origin (spβ-NGF) increases CL vascularization and level of mRNA expression of steroidogenic enzymes during the early stage of Corpus Luteum development in llamas

The objectives of the study were to determine the effect of seminal plasma β-NGF on Corpus Luteum morphology and function and level of mRNA expression of steroidogenic enzymes. Llamas were assigned (n = 12/per group) to receive an intramuscular dose of: (a) 1 ml phosphate buffered saline (PBS), (b)...

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Bibliographic Details
Published in:Theriogenology 2017-11, Vol.103, p.69-75
Main Authors: Silva, M., Ulloa-Leal, C., Valderrama, X.P., Bogle, O.A., Adams, G.P., Ratto, M.H.
Format: Article
Language:English
Subjects:
Animals
Camelids, New World - physiology
Corpus Luteum
Corpus Luteum - blood supply
Corpus Luteum - drug effects
Cytochrome P-450 Enzyme System - genetics
Cytochrome P-450 Enzyme System - metabolism
Female
Gene Expression Regulation, Enzymologic - drug effects
Gonadotropin-Releasing Hormone - pharmacology
Llama
Male
Nerve Growth Factor - pharmacology
OIF
Phosphoproteins - genetics
Phosphoproteins - metabolism
Pregnancy
RNA, Messenger - genetics
RNA, Messenger - metabolism
Semen - chemistry
Semen - metabolism
Spβ-NGF
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Summary:The objectives of the study were to determine the effect of seminal plasma β-NGF on Corpus Luteum morphology and function and level of mRNA expression of steroidogenic enzymes. Llamas were assigned (n = 12/per group) to receive an intramuscular dose of: (a) 1 ml phosphate buffered saline (PBS), (b) 5 μg gonadorelin acetate (GnRH), or (c) 1.0 mg of purified llama spβ-NGF. Ovaries were examined by transrectal B-mode ultrasonography from treatment to ovulation (Day 0 = treatment). B mode/Power Doppler ultrasonography and blood samples collection were performed at Days 4, 8 and 10 (n = 3 llamas per treatment group/per time point) to determine CL diameter, vascularization and plasma progesterone concentration respectively. Plasma progesterone concentration was analyzed in all llamas at Day 0. Then females were submitted to ovariectomy at Days 4, 8 and 10 (n = 3 llamas/treatment/time), CL was removed to determine vascular area, proportion of luteal cells and CYP11A1/P450scc and STAR expression by RT-PCR. Ovulation was similar between llamas treated with GnRH or spβ-NGF and CL diameter did not differ between GnRH or spβ-NGF groups by Day 4, 8 or 10. Vascularization area of the CL was higher (P 
ISSN:0093-691X
1879-3231
DOI:10.1016/j.theriogenology.2017.07.041