In vitro biofilm models to study dental caries: a systematic review

The aim of this systematic review is to characterize and discuss key methodological aspects of in vitro biofilm models for caries-related research and to verify the reproducibility and dose-response of models considering the response to anti-caries and/or antimicrobial substances. Inclusion criteria...

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Bibliographic Details
Published in:Biofouling (Chur, Switzerland) Switzerland), 2017-09, Vol.33 (8), p.661-675
Main Authors: Maske, T. T., van de Sande, F. H., Arthur, R. A., Huysmans, M. C. D. N. J. M., Cenci, M. S.
Format: Article
Language:English
Subjects:
Anti-Infective Agents - pharmacology
Antiinfectives and antibacterials
artificial caries
Biofilm models
Biofilms
Biofilms - drug effects
Biofilms - growth & development
caries-like lesion
Colony Count, Microbial
Culture Media
Dental caries
Dental Caries - microbiology
Dental Caries - prevention & control
Dental materials
Dose-Response Relationship, Drug
Dynamic models
Humans
In vitro methods and tests
in vitro studies
Lesions
Models, Biological
pH effects
Reproducibility
Reproducibility of Results
Saliva, Artificial
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Summary:The aim of this systematic review is to characterize and discuss key methodological aspects of in vitro biofilm models for caries-related research and to verify the reproducibility and dose-response of models considering the response to anti-caries and/or antimicrobial substances. Inclusion criteria were divided into Part I (PI): an in vitro biofilm model that produces a cariogenic biofilm and/or caries-like lesions and allows pH fluctuations; and Part II (PII): models showing an effect of anti-caries and/or antimicrobial substances. Within PI, 72.9% consisted of dynamic biofilm models, while 27.1% consisted of batch models. Within PII, 75.5% corresponded to dynamic models, whereas 24.5% corresponded to batch models. Respectively, 20.4 and 14.3% of the studies reported dose-response validations and reproducibility, and 32.7% were classified as having a high risk of bias. Several in vitro biofilm models are available for caries-related research; however, most models lack validation by dose-response and reproducibility experiments for each proposed protocol.
ISSN:0892-7014
1029-2454
DOI:10.1080/08927014.2017.1354248