Synthesis of the N-terminal of the Ice Nucleation Protein Gene of Pseudomonas syringae by Assembly PCR

The construction of a synthetic gene coding for the N-terminal of the ice nucleation protein of Pseudomonas syringae using assembly polymerase chain reaction was described. The Inak-n gene was assembled from 34 overlapping oligonucleotides in a single step and amplified by PCR using specific cloning...

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Bibliographic Details
Published in:Biotechnology (Faisalābād, Pakistan) Pakistan), 2005-07, Vol.4 (3), p.187-193
Main Authors: Sarhan, MAA, Musa, M, Nor, N M, Zainuddin, Z F
Format: Article
Language:English
Subjects:
Escherichia coli
Pseudomonas syringae
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Summary:The construction of a synthetic gene coding for the N-terminal of the ice nucleation protein of Pseudomonas syringae using assembly polymerase chain reaction was described. The Inak-n gene was assembled from 34 overlapping oligonucleotides in a single step and amplified by PCR using specific cloning primers. Expression of this gene in E. coli, in the form of a fusion protein, was confirmed by Western blotting. This synthetic gene would be a useful tool for the display of antigens on the surface of bacterial cells.
ISSN:1682-296X
1682-2978
DOI:10.3923/biotech.2005.187.193