Antibiotic susceptibilities and prevalence of Methicillin resistant Staphylococcus aureus (MRSA) isolated from bovine milk in Pakistan

• Published in: Acta tropica 2017-12, Vol.176, p.168-172
• Main Authors: Aqib, Amjad Islam, Ijaz, Muhammad, Anjum, Aftab Ahmad, Malik, Muhammad Abdul Rauf, Mehmood, Khalid, Farooqi, Shahid Hussain, Hussain, Kashif
• Format: Article
• Language: English
• Subjects: Animals; Anti-Bacterial Agents - pharmacology; Buffalo; Cattle; Drug Resistance, Bacterial; Female; In-vitro therapeutic efficacy; Methicillin resistant Staphylococcus aureus (MRSA); Methicillin-Resistant Staphylococcus aureus - isolation & purification; Microbial Sensitivity Tests; Milk; Milk - microbiology; Pakistan - epidemiology; Polymerase Chain Reaction; Prevalence; Risk factors
• ISSN: 0001-706X; 1873-6254
• DOI: 10.1016/j.actatropica.2017.08.008

•Staphylococcus aureus (S. aureus) influences are well-known to cause subclinical, clinical, and chronic mastitis.•The genetic capabilities of methicillin-resistant S. aureus (MRSA) strains enhance pathogenesis of S. aureus in mastitis.•Bovine milk samples from district Faisalabad presented 34% of MRSA prevalence, with 30% from cattle and 38% from buffalo.•Ciprofloxcin, Moxifloxacin, Linezolid, and Trimethoprim plus Sulphamethoxazole drugs showed highest efficacy against MRSA. The study was designed to investigate bovine milk for prevalence of an emerging zoonotic pathogen Methicillin resistant Staphylococcus aureus (MRSA), and in-vitro therapeutic response of various antibiotics against MRSA. Nine hundred (900) milk samples were collected (half from cattle and half from buffalo) from private and public farms located in various tehsils of district Faisalabad, using the convenient sampling method. Milk samples were put to biochemical identification of Staphylococcus aureus and later oxacilline disk sensitivity testing for confirmation of MRSA. The MRSA isolates were confirmed by PCR targeting mecA gene in Staphylococcus aureus. The study found 34% prevalence of MRSA in overall bovine milk from district Faisalabad with 30% and 38% prevalence in cattle and buffalo, respectively. Tehsil Samundari presented comparatively higher MRSA prevalence followed by tehsil Jaranwala and tehsil Faisalabad. However, there was non-significant difference of MRSA prevalence between cattle and buffalo, and among different tehsils. All assumed risk factors except specie were significantly associated with mastitis spread. The in-vitro drug trial against MRSA from buffalo milk presented 100% efficacy of Ciprofloxcin, Moxifloxacin, Linezolid, and Trimethoprim plus Sulphamethoxazole combination, followed by Gentamicin and Levofloxacin presenting 90%, and Amikacin becoming 80% efficacious against MRSA from buffalo milk. The MRSA isolates of cattle milk presented similar pattern with some variations of higher susceptibility against Oxytetracycline, and Fusidic acid. The conclusion of the study states uniform prevalence of MRSA in cattle and buffalo milk in study area having assumed risk factors positively associated with disease spread, while Ciprofloxcin, Moxifloxacin, Linezolid, and Trimethoprim plus Sulphamethoxazole drugs showed the highest efficacy to combat this pathogen.