The inhibitory effect of 5,7‐DMF on pancreatic sphere‐forming cell function mediated by FoxM1 gene expression

Pancreatic cancer is one of the major human malignant tumors severely endangering human health and life with high mortality due to the concealment of early symptoms and lack of effective therapies during advanced stages. The identification of pancreatic cancer stem cell functions has been as importa...

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Bibliographic Details
Published in:Journal of cellular biochemistry 2018-02, Vol.119 (2), p.1855-1865
Main Authors: Zeng, Deyu, Ma, Jian, Li, Rongrong, Yang, Jianfeng, Yin, Xianli
Format: Article
Language:English
Subjects:
5,7‐DMF
Biomarkers
Cancer
Cancer therapies
Cell migration
Forming
FoxM1
Gene expression
Inhibition
Pancreatic cancer
Sox2
Sox2 protein
sphere‐forming cell
stem cell
Stem cells
Tumors
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Summary:Pancreatic cancer is one of the major human malignant tumors severely endangering human health and life with high mortality due to the concealment of early symptoms and lack of effective therapies during advanced stages. The identification of pancreatic cancer stem cell functions has been as important strategy for understanding of pancreatic cancer biology and novel drug and therapy development. In the present study, we successfully isolated the pancreatic sphere‐forming cells from pancreatic cancer cell line PANC‐1 by sphere‐forming method and we found that the sphere‐forming ability and the cell migration rate of pancreatic sphere‐forming cells were significantly inhibited by 5,7‐DMF treatment, which was supported by the corresponding changes of several EMT biomarkers after being treated with 5,7‐DMF. Moreover, we revealed here that the inhibition of pancreatic sphere‐forming cells was mediated by the expression of FoxM1 gene, and also the expression of SOX2 gene was regulated by FoxM1 in pancreatic sphere‐forming cells and involved in the inhibitory role of 5,7‐DMF. These results provided important basis for the application of 5,7‐DMF as a novel drug candidate for the pancreatic cancer treatment. The inhibition of pancreatic cancer stem cells was mediated by the expression of FoxM1 gene, and also the expression of SOX2 gene was regulated by FoxM1 in pancreatic cancer stem cells and involved in the inhibitory role of 5,7‐DMF. These results provided important basis for the application of 5,7‐DMF as a novel drug candidate for the pancreatic cancer treatment.
ISSN:0730-2312
1097-4644
DOI:10.1002/jcb.26346